2. Academic Validation
  3. N-glycosylation of serum proteins in adult type 1 diabetes mellitus exposes further changes compared to children at the disease onset

N-glycosylation of serum proteins in adult type 1 diabetes mellitus exposes further changes compared to children at the disease onset

  • Clin Chim Acta. 2023 Mar 15:543:117298. doi: 10.1016/j.cca.2023.117298.
Matej Nemčić 1,  Marko Tijardović 2,  Najda Rudman 3,  Tomislav Bulum 4,  Martina Tomić 5,  Branimir Plavša 6,  Sandra Vučković Rebrina 7,  Marijana Vučić Lovrenčić 8,  Lea Duvnjak 9,  Grant Morahan 10,  Olga Gornik 11
Affiliations

Affiliations

  • 1 Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia. Electronic address: mnemcic@pharma.unizg.hr.
  • 2 Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia. Electronic address: marko.tijardovic@gmail.com.
  • 3 Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia. Electronic address: nrudman@pharma.unizg.hr.
  • 4 Department of Endocrinology, Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, Merkur University Hospital, Dugi dol 4A, 10000 Zagreb, Croatia. Electronic address: tomislav.bulum@kb-merkur.hr.
  • 5 Department of Ophthalmology, Vuk Vrhovac University Clinic, Merkur University Hospital, Dugi dol 4A, 10000 Zagreb, Croatia. Electronic address: martina.tomic@idb.hr.
  • 6 Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia. Electronic address: bplavsa@pharma.unizg.hr.
  • 7 Department of Endocrinology, Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, Merkur University Hospital, Dugi dol 4A, 10000 Zagreb, Croatia. Electronic address: svuckovic@idb.hr.
  • 8 Department of Clinical Chemistry and Laboratory Medicine, Merkur University Hospital, Zajčeva ul. 19, 10000 Zagreb, Croatia. Electronic address: mvuciclovrencic@gmail.com.
  • 9 Department of Endocrinology, Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, Merkur University Hospital, Dugi dol 4A, 10000 Zagreb, Croatia. Electronic address: lduvnjak@idb.hr.
  • 10 Centre for Diabetes Research, The Harry Perkins Institute for Medical Research, 6 Verdun St, Nedlands WA 6009, Perth, Australia; Australian Centre for Accelerating Diabetes Innovations, University of Melbourne, Swanston St, Parkville, VIC 3052, Australia. Electronic address: grant.morahan@perkins.org.au.
  • 11 Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia. Electronic address: ogornik@pharma.unizg.hr.
PMID: 36925056 DOI: 10.1016/j.cca.2023.117298
Abstract

Objective: Previously we have shown that plasma protein N-glycosylation is changed in children at the onset of type 1 diabetes. In this study, we aim to identify N-glycan changes in adults with T1DM, compare them to those in children, and investigate their associations with disease duration, complications, glycaemic status, and smoking.

Methods: Serum protein N-glycans from 200 adults with type 1 diabetes and 298 healthy controls were analysed using ultra-high performance liquid chromatography and divided into 39 directly measured glycan groups from which 16 derived traits were calculated.

Results: Compared to healthy controls, subjects with type 1 diabetes showed differences in 19 glycan groups and a decrease in monogalactosylated, an increase in digalactosylated, monosialylated, and antennary fucosylated derived traits, from which changes in monogalactosylation and seven directly measured traits overlapped with previously reported in children. Changes in four directly measured and two derived traits previously seen in children were not detected in adults. HbA1c was positively associated with sialylated and highly branched structures, whereas N-glycome was not influenced by disease duration or diabetic complications.

Conclusions: Our results suggest potential N-glycome involvement in different stages of type 1 diabetes, including processes underlying its development, the disease itself, as well as those occurring after disease establishment.

Keywords

Diabetes mellitus complications; HbA1c; Serum protein N-glycosylation; Smoking; Type 1 diabetes mellitus.

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