Prooxidant capacity of phenolic acids defines antioxidant potential

Phenolic acids derived from vegetables, fruits and beverages are considered abundant sources of natural Antioxidants consumed in the human diet. In addition to having well-known antioxidant activity, phenolic acids also exhibit pro-oxidant activity under selected conditions. We hypothesized that the availability of extracellular H₂O₂ derived from phenolic acid autoxidation will diffuse across cell membranes to participate as a messenger molecule to activate intracellular redox signaling in response to oxidative stress. We report on the relative activity of structurally different phenolic acids to generate specific changes in the extracellular - intracellular H₂O₂ flux that induces intracellular redox signaling corresponding to a function to reduce intracellular oxidative stress. HyPer-3 methodology was used to measure increases in intracellular H₂O₂ in differentiated Caco-2 intestinal cells in response to phenolic acid autoxidation and changes in extracellular H₂O₂ production. The potential for different phenolic acids to autoxidize and generate H₂O₂ was dependent on the structure and concentration of phenolic acid. Activation of nuclear factor erythroid 2-related factor (Nrf2) cell signaling was enhanced (p < 0.05) by phenolic acid induced H₂O₂ production, and mitigated when present along with catalase (p < 0.05), or, alternatively by blocking AQP3 function (p < 0.05) using DFP00173 as the AQP3 inhibitor. The relative capacity of phenolic acids to generate H₂O₂ on autoxidation was structure specific and corresponded to the level of Nrf2 cell signaling in differentiated Caco-2 epithelial cells. The Nrf2-Keap1 response paralleled the extent of reduced oxidative stress observed in differentiated Caco-2 cells determined by DCFH-DA.

Aquaporin; Caco-2 cells; H(2)O(2); HyPer-3; Nrf2 cell signaling; Phenolic acids.