2. Academic Validation
  3. Silicate Nanoplatelets Promotes Neuronal Differentiation of Neural Stem Cells and Restoration of Spinal Cord Injury

Silicate Nanoplatelets Promotes Neuronal Differentiation of Neural Stem Cells and Restoration of Spinal Cord Injury

  • Adv Healthc Mater. 2023 May 4;e2203051. doi: 10.1002/adhm.202203051.
Yifan Wang 1 2 3 4,  Tengfei Zhao 1 2 3 4,  Yiren Jiao 5,  Hanxu Huang 1 2 3 4,  Yongxiang Zhang 1 2 3 4,  Ao Fang 6,  Xuhua Wang 6,  Yanling Zhou 7,  Haochen Gu 8,  Qionghua Wu 1 2 3 4,  Jiang Chang 5 7,  Fangcai Li 1 2 3 4,  Kan Xu 1 2 3 4
Affiliations

Affiliations

  • 1 Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University, School of Medicine, No.88 Jiefang Road, Hangzhou City, 310009, P. R. China.
  • 2 Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, 310009, P. R. China.
  • 3 Key Laboratory Research of Motor System Disease and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, 310009, P. R. China.
  • 4 Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou City, Zhejiang Province, 310009, P. R. China.
  • 5 Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325000, P. R. China.
  • 6 NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang Province, 310003, P. R. China.
  • 7 Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, P. R. China.
  • 8 School of Medicine, Zhejiang University, Hangzhou City, 310009, P. R. China.
PMID: 37141006 DOI: 10.1002/adhm.202203051
Abstract

Neural stem cell (NSC) transplantation has been suggested as a promising therapeutic strategy to replace lost neurons after spinal cord injury (SCI). However, the low survival rate and neuronal differentiation efficiency of implanted NSCs within the lesion cavity limit the application. Furthermore, it is difficult for transplanted cells to form connections with host cells. Thus, effective and feasible methods to enhance the efficacy of cell transplantation are needed. In this study, the effect of Laponite nanoplatelets, a type of silicate nanoplatelets, on stem cell therapy is explored. Laponite nanoplatelets can induce the neuronal differentiation of NSCs in vitro within five days, and RNA Sequencing and protein expression analysis demonstrated that the NF-κB pathway is involved in this process. Moreover, histological results revealed that Laponite nanoplatelets can increase the survival rate of transplanted NSCs and promote NSCs to differentiate into mature neurons. Finally, the formation of connections between transplanted cells and host cells is confirmed by axon tracing. Hence, Laponite nanoplatelets, which drove neuronal differentiation and the maturation of NSCs both in vitro and in vivo, can be considered a convenient and practical biomaterial to promote repair of the injured spinal cord by enhancing the efficacy of NSC transplantation.

Keywords

differentiation; laponite; neural stem cells; spinal cord injury; stem cell therapy.

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