2. Academic Validation
  3. β-asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress

β-asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress

  • Cancer Med. 2023 Jun 12. doi: 10.1002/cam4.6059.
Bo Liu 1 2 3 4,  Weichao Dan 2 3 4,  Yi Wei 2 3 4,  Yan Zhang 1,  Chi Wang 2 3 4,  Yuzeshi Lei 2 3 4,  Tao Hou 2 3 4,  Yong Zhang 1,  Ye Gao 5
Affiliations

Affiliations

  • 1 Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, P. R. China.
  • 2 Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, P. R. China.
  • 3 Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, P. R. China.
  • 4 Key Laboratory for Tumor Precision Medicine of Shaanxi Province, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, P. R. China.
  • 5 Department of Emergency, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, P. R. China.
PMID: 37306628 DOI: 10.1002/cam4.6059
Abstract

Background: β-asarone (β-as), a compound extracted from Acorus calamus, has been found to have Anticancer effects on a variety of human cancers. However, the potential effect of β-as on bladder Cancer (BCa) remains unknown.

Methods: After exposure to β-as, migration, invasion, and epithelial-mesenchymal transition (EMT) of BCa were determined by wound healing, transwell, and Western blot assays. Expression of proteins involved in the EMT and ER stress were explored by Western blot assays. Nude mouse xenograft model was served as the model system in vivo.

Results: The migration, invasion, and EMT of BCa were significantly inhibited after β-as treatment. Further experiments revealed that endoplasmic reticulum (ER) stress is involved in β-as-mediated metastasis inhibition. In addition, β-as significantly up-regulated activating transcription factor 6 (ATF6), a branch of ER stress, and promoted its Golgi cleavage and nuclear localization. ATF6 silencing attenuated β-as-mediated metastasis and EMT inhibition in BCa cells.

Conclusion: Our data suggests that β-as inhibits migration, invasion, and EMT of BCa by activating the ATF6 branch of ER stress. Thus, β-as represents a potential candidate for BCa treatment.

Keywords

ATF6; EMT; ER stress; invasion; migration; β-asarone.

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