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  3. Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids

Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids

  • STAR Protoc. 2023 Jul 9;4(3):102431. doi: 10.1016/j.xpro.2023.102431.
Kimberley Ober 1,  Floris J M Roos 2,  Gilles S van Tienderen 3,  Kübra Köten 3,  Annelot Klaassen 3,  Wunan Mi 3,  Luc J W van der Laan 3,  Monique M A Verstegen 4
Affiliations

Affiliations

  • 1 Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands. Electronic address: k.ober@erasmusmc.nl.
  • 2 Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge and NIHR Biomedical Research Centre, Cambridge, UK; Department of Surgery, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK.
  • 3 Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands.
  • 4 Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands. Electronic address: m.verstegen@erasmus.nl.
PMID: 37432852 DOI: 10.1016/j.xpro.2023.102431
Abstract

Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than branching duct morphology. Here, we present a protocol to establish branching morphogenesis in cholangiocyte and cholangiocarcinoma organoids. We describe steps for the initiation, maintenance, and expansion of intrahepatic cholangiocyte organoids branching morphology. This protocol enables the study of organ-specific and mesenchymal-independent branching morphogenesis and provides an improved model to study biliary function and diseases. For complete details on the use and execution of this protocol, please refer to Roos et al. (2022).1.

Keywords

Cancer; Cell Biology; Developmental biology; Organoids.

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