2. Academic Validation
  3. Low intensity pulsed ultrasound ameliorates Adriamycin-induced chronic renal injury by inhibiting ferroptosis

Low intensity pulsed ultrasound ameliorates Adriamycin-induced chronic renal injury by inhibiting ferroptosis

  • Redox Rep. 2023 Dec;28(1):2251237. doi: 10.1080/13510002.2023.2251237.
Zhi-Qiang Ouyang 1,  Li-Shi Shao 2,  Wei-Peng Wang 3,  Teng-Fei Ke 4,  Dong Chen 5,  Guang-Rong Zheng 1,  Xi-Rui Duan 4,  Ji-Xiang Chu 4,  Yu Zhu 4,  Lu Yang 4,  Hai-Yan Shan 1,  Lin Huang 3,  Cheng-de Liao 1
Affiliations

Affiliations

  • 1 Department of Radiology, Yan` an Hospital of Kunming City (Yanan Hospital Affiliated to Kunming Medical University), Kunming, People's Republic of China.
  • 2 Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People's Republic of China.
  • 3 School of Electronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.
  • 4 Department of Radiology, Yunnan Cancer Hospital (The Third Affiliated Hospital of Kunming Medical University), Kunming, People's Republic of China.
  • 5 Department of Ultrasound, Yunnan Cancer Hospital (The Third Affiliated Hospital of Kunming Medical University), Kunming, People's Republic of China.
PMID: 37652897 DOI: 10.1080/13510002.2023.2251237
Abstract

Objective: It is very important to develop a new therapeutic strategy to cope with the increasing morbidity and mortality of chronic kidney disease (CKD). As a kind of physical therapy, low intensity pulsed ultrasound (LIPUS) has remarkable anti-inflammatory and repair-promoting effects and is expected to become a new therapeutic method for CKD. This study aims to clarify the treatment effect of LIPUS on CKD-related renal inflammation and fibrosis, and to further explore the potential signal network of LIPUS treatment for ameliorating chronic renal injury.

Methods: A rat model simulating the progress of CKD was established by twice tail-vein injection of Adriamycin (ADR). Under anesthesia, bilateral kidneys of CKD rats were continuously stimulated by LIPUS for four weeks. The parameters of LIPUS were 1.0 MHz, 60 mW/cm2, 50% duty cycle and 20 min/d.

Results: LIPUS treatment effectively inhibited ADR-induced renal inflammation and fibrosis, and improved CKD-related to oxidative stress and Ferroptosis. In addition, the therapeutic effect of LIPUS is closely related to the regulation of TGF-β1/Smad and Nrf2/keap1/HO-1 signalling pathways.

Discussion: This study provides a new direction for further mechanism research and lays an important foundation for clinical trials.

Keywords

Chronic kidney disease; Ferroptosis; Fibrosis; Inflammation; Low intensity pulsed ultrasound; Nrf2/keap1/HO-1 pathway; Oxidative stress; TGF-β1/Smad pathway.

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