1. Academic Validation
  2. Role of thrombospondin-1 in high-salt-induced mesenteric artery endothelial impairment in rats

Role of thrombospondin-1 in high-salt-induced mesenteric artery endothelial impairment in rats

  • Acta Pharmacol Sin. 2024 Mar;45(3):545-557. doi: 10.1038/s41401-023-01181-9.
Fang-Fang Xu # 1 2 Fan Zheng # 1 Ye Chen # 3 Yang Wang # 4 Shao-Bo Ma 1 Weng Ding 1 Le-Sha Zhang 1 Ji-Zheng Guo 1 Chang-Bo Zheng 5 Bing Shen 6
Affiliations

Affiliations

  • 1 School of Basic Medical Sciences, Anhui Medical University, Hefei, 230032, China.
  • 2 Department of Pharmacy, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, China.
  • 3 Department of Pathology, The First Affiliated Hospital of Anhui Medical University, Hefei, 230032, China.
  • 4 Department of Otolaryngology-Head and Neck Surgery, Lu'an People's Hospital, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an, 237000, China.
  • 5 School of Pharmaceutical Science, Kunming Medical University, Kunming, 650500, China. zhengchangbo@kmmu.edu.cn.
  • 6 School of Basic Medical Sciences, Anhui Medical University, Hefei, 230032, China. shenbing@ahmu.edu.cn.
  • # Contributed equally.
Abstract

The matrix glycoprotein thrombospondin-1 (THBS1) modulates nitric oxide (NO) signaling in endothelial cells. A high-salt diet induces deficiencies of NO production and bioavailability, thereby leading to endothelial dysfunction. In this study we investigated the changes of THBS1 expression and its pathological role in the dysfunction of mesenteric artery endothelial cells (MAECs) induced by a high-salt diet. Wild-type rats, and wild-type and Thbs1-/- mice were fed chow containing 8% w/w NaCl for 4 weeks. We showed that a high salt diet significantly increased THBS1 expression and secretion in plasma and MAECs, and damaged endothelium-dependent vasodilation of mesenteric resistance arteries in wild-type Animals, but not in Thbs1-/- mice. In rat MAECs, we demonstrated that a high salt environment (10-40 mM) dose-dependently increased THBS1 expression accompanied by suppressed endothelial nitric oxide synthase (eNOS) and phospho-eNOS S1177 production as well as NO release. Blockade of transforming growth factor-β1 (TGF-β1) activity by a TGF-β1 inhibitor SB 431542 reversed THBS1 up-regulation, rescued the eNOS decrease, enhanced phospho-eNOS S1177 expression, and inhibited SMAD4 translocation to the nucleus. By conducting dual-luciferase reporter experiments in HEK293T cells, we demonstrated that SMAD4, a transcription promoter, upregulated Thbs1 transcription. We conclude that THBS1 contributes to endothelial dysfunction in a high-salt environment and may be a potential target for treatment of high-salt-induced endothelium dysfunction.

Keywords

Smad4; TGF-β1; eNOS; endothelial cells; high-salt diet; thrombospondin-1.

Figures
Products