Identification of Novel -Glucosidase Inhibitors from Syzygium jambos (L.) Using Spectroscopy and Molecular Docking

Fruits of Syzygium jambos (L.) are recognized as a "food", exhibiting significant antidiabetic activities. However, the α-glucosidase inhibition of the components from Syzygium jambos (L.) have not yet been investigated. In this study, a total of 14 compounds were isolated from Syzygium jambos (L.) Alston, eight of which showed significant inhibitory effects on α-glucosidase, with IC₅₀ values in the range of 0.011-0.665 mM. Notably, compounds 1-3 (IC₅₀: 0.013, 0.011 and 0.030 mM, respectively) exhibited much stronger activity than acarbose (IC₅₀: 2.329 ± 0.109 mM). The Enzyme kinetics study indicated that compound 1 was an uncompetitive inhibitor, and compounds 2-8 were mixed-type inhibitors. Moreover, the interactions between compounds and α-glucosidase were investigated by molecular docking, which further revealed that the number of olefin double bonds and 2-COOH of heptadeca-phenols had a notable effect on the α-glucosidase inhibitory activity. This study demonstrated that Syzygium jambos (L.) fruit might serve as a functional food for the prevention of diabetes mellitus.

Alkylphenol derivatives; Molecular docking; Syzygium jambos (L.) Alston fruit; Type 2 diabetes; α-glucosidase inhibition.