2. Academic Validation
  3. Aiouea padiformis extract exhibits anti-inflammatory effects by inhibiting the ATPase activity of NLRP3

Aiouea padiformis extract exhibits anti-inflammatory effects by inhibiting the ATPase activity of NLRP3

  • Sci Rep. 2024 Mar 4;14(1):5237. doi: 10.1038/s41598-024-55651-z.
Sumin Lee # 1 2,  Qianying Ye # 3,  Hyeyun Yang 1 2,  Sojung Lee 1 2,  YeJi Kim 1 2,  Nahyun Lee 4,  Darwin Gonzalez-Cox 5,  Dong-Keun Yi 6,  Soo-Yong Kim 6,  Sangho Choi 6,  Taesoo Choi 7,  Man S Kim 4,  Seong Su Hong 8,  Chun Whan Choi 9,  Yoonsung Lee 10,  Yong Hwan Park 11 12
Affiliations

Affiliations

  • 1 Department of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
  • 2 Department of Biomedical Sciences, Graduate School of Ajou University, Suwon, Republic of Korea.
  • 3 Department of Biomedical Science and Technology, Kyung Hee University, Seoul, 02447, Republic of Korea.
  • 4 Clinical Research Institute, Kyung Hee University Hospital at Gangdong, School of Medicine, Kyung Hee University, Seoul, 05278, Republic of Korea.
  • 5 Herbarium of National Autonomous University of Nicaragua at Leon, Leon, 21000, Nicaragua.
  • 6 International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
  • 7 Department of Urology, School of Medicine, Kyung Hee University, Seoul, 05278, Republic of Korea.
  • 8 Natural Product Research Team, Gyeonggi Bio-Center, Suwon, Republic of Korea.
  • 9 Natural Product Research Team, Gyeonggi Bio-Center, Suwon, Republic of Korea. cwchoi@gbsa.or.kr.
  • 10 Clinical Research Institute, Kyung Hee University Hospital at Gangdong, School of Medicine, Kyung Hee University, Seoul, 05278, Republic of Korea. ylee3699@khu.ac.kr.
  • 11 Department of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea. parky5@ajou.ac.kr.
  • 12 Department of Biomedical Sciences, Graduate School of Ajou University, Suwon, Republic of Korea. parky5@ajou.ac.kr.
  • # Contributed equally.
PMID: 38433281 DOI: 10.1038/s41598-024-55651-z
Abstract

Inflammation is implicated as a cause in many diseases. Most of the anti-inflammatory agents in use are synthetic and there is an unmet need for natural substance-derived anti-inflammatory agents with minimal side effects. Aiouea padiformis belongs to the Lauraceae family and is primarily found in tropical regions. While some members of the Aiouea genus are known to possess anti-inflammatory properties, the anti-inflammatory properties of Aiouea padiformis extract (AP) have not been investigated. In this study, we aimed to examine the anti-inflammatory function of AP through the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome and elucidate the underlying mechanisms. Treatment with AP inhibited the secretion of interleukin-1 beta (IL-1β) mediated by NLRP3 inflammasome in J774A.1 and THP-1 cells without affecting the viability. In addition, AP treatment did not influence NF-κB signaling, potassium efflux, or intracellular Reactive Oxygen Species (ROS) production-all of which are associated with NLRP3 inflammasome activation. However, intriguingly, AP treatment significantly reduced the ATPase activity of NLRP3, leading to the inhibition of ASC oligomerization and speck formation. Consistent with cellular experiments, the anti-inflammatory property of AP in vivo was also evaluated using an LPS-induced inflammation model in zebrafish, demonstrating that AP hinders NLRP3 inflammasome activation.

Keywords

Aiouea padiformis; Anti-inflammation; Lauraceae; NLRP3 inflammasome; Plant extracts.

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