2. Academic Validation
  3. Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast

Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast

  • Nat Commun. 2024 Apr 23;15(1):3437. doi: 10.1038/s41467-024-47746-y.
Lihui Jiang # 1,  Xiaoyan Zhang # 1,  Yiting Zhao # 1 2,  Haiyan Zhu 1,  Qijing Fu 1,  Xinqi Lu 1,  Wuying Huang 1,  Xinyue Yang 1,  Xuan Zhou 1,  Lixia Wu 1,  Ao Yang 1,  Xie He 1,  Man Dong 1,  Ziai Peng 1,  Jing Yang 1 3 4,  Liwei Guo 1 3 4,  Jiancheng Wen 5,  Huichuan Huang 1 3 4,  Yong Xie 1 3 4,  Shusheng Zhu 1 3 4,  Chengyun Li 1 3 4,  Xiahong He 6,  Youyong Zhu 1 3 4,  Jiří Friml 7,  Yunlong Du 8 9 10
Affiliations

Affiliations

  • 1 College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.
  • 2 Shanxi Agricultural University/Shanxi Academy of Agricultural Sciences. The Industrial Crop Institute, Fenyang, 032200, China.
  • 3 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
  • 4 Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
  • 5 Rice Research Institute, Yunnan Agricultural University, Kunming, 650201, China.
  • 6 Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, China.
  • 7 Institute of Science and Technology Austria (IST Austria), Klosterneuburg, Austria.
  • 8 College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China. yunlongdu@aliyun.com.
  • 9 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China. yunlongdu@aliyun.com.
  • 10 Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China. yunlongdu@aliyun.com.
  • # Contributed equally.
PMID: 38653755 DOI: 10.1038/s41467-024-47746-y
Abstract

Phytoalexin sakuranetin functions in resistance against rice blast. However, the mechanisms underlying the effects of sakuranetin remains elusive. Here, we report that rice lines expressing resistance (R) genes were found to contain high levels of sakuranetin, which correlates with attenuated endocytic trafficking of plasma membrane (PM) proteins. Exogenous and endogenous sakuranetin attenuates the endocytosis of various PM proteins and the Fungal effector PWL2. Moreover, accumulation of the avirulence protein AvrCO39, resulting from uptake into rice cells by Magnaporthe oryzae, was reduced following treatment with sakuranetin. Pharmacological manipulation of clathrin-mediated endocytic (CME) suggests that this pathway is targeted by sakuranetin. Indeed, attenuation of CME by sakuranetin is sufficient to convey resistance against rice blast. Our data reveals a mechanism of rice against M. oryzae by increasing sakuranetin levels and repressing the CME of pathogen effectors, which is distinct from the action of many R genes that mainly function by modulating transcription.

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