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  3. Transient autophagy inhibition strengthened postharvest tomato (Solanum lycopersicum) resistance against Botrytis cinerea through curtailing ROS-induced programmed cell death

Transient autophagy inhibition strengthened postharvest tomato (Solanum lycopersicum) resistance against Botrytis cinerea through curtailing ROS-induced programmed cell death

  • Food Chem. 2024 May 25:454:139811. doi: 10.1016/j.foodchem.2024.139811.
Quan Ma 1,  Dong Li 2,  Yicheng Ren 1,  Yanpei Chen 1,  Jing Huang 1,  Bin Wu 3,  Qingqing Wang 1,  Zisheng Luo 4
Affiliations

Affiliations

  • 1 Zhejiang University, College of Biosystems Engineering and Food Science, The Rural Development Academy, Zhejiang University, Hangzhou 310058, China.
  • 2 Zhejiang University, College of Biosystems Engineering and Food Science, The Rural Development Academy, Zhejiang University, Hangzhou 310058, China. Electronic address: dong_li@zju.edu.cn.
  • 3 Institute of Agro-products Storage and Processing & Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products, Xinjiang Academy of Agricultural Science, Urumqi 830091, China.
  • 4 Zhejiang University, College of Biosystems Engineering and Food Science, The Rural Development Academy, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agri-Food Processing, Zhejiang University, Hangzhou 310058, China; Ningbo Innovation Center, Zhejiang University, Ningbo 315100, China. Electronic address: luozisheng@zju.edu.cn.
PMID: 38820631 DOI: 10.1016/j.foodchem.2024.139811
Abstract

Autophagy (AU) and programmed cell death (PCD) are dynamically regulated during tomato fruit defense against Botrytis cinerea, which are also manipulated by pathogenic effectors to promote colonization. Present study demonstrated that the enhanced defense induced by transient inhibition on AU by hydroxychloroquine (HCQ) facilitated the restriction of B. cinerea lesion on postharvest tomato. Pre-treatment of 2 mM (16.08 ± 3.42 cm at 7 d) and 6 mM (7.80 ± 2.39 cm at 7 d) HCQ inhibited the lesion development of B. cinerea compared with Mock treatment (50.02 ± 7.69 cm at 7 d). Transient inhibition of AU induced expression of Fungal defense and transcriptional regulation related genes, but attenuated Reactive Oxygen Species (ROS) burst gene expression. The ROS-induced PCD was compromised by HCQ with promoted ROS scavenging. The transient pre-treatment of HCQ slightly inhibited AU which triggered the feedback loop that enhanced the autophagic activity defensing against B. cinerea Infection.

Keywords

Autophagy; Gray mold; Programmed cell death; ROS; Tomato.

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