2. Academic Validation
  3. Heat stress induces calcium dyshomeostasis to subsequent cognitive impairment through ERS-mediated apoptosis via SERCA/PERK/eIF2α pathway

Heat stress induces calcium dyshomeostasis to subsequent cognitive impairment through ERS-mediated apoptosis via SERCA/PERK/eIF2α pathway

  • Cell Death Discov. 2024 Jun 11;10(1):280. doi: 10.1038/s41420-024-02047-7.
Hongxia Li # 1,  Wenlan Pan # 2,  Chenqi Li # 1 3,  Mengyu Cai 1,  Wenjing Shi 1,  Zifu Ren 1,  Hongtao Lu 4,  Qicheng Zhou 5,  Hui Shen 6
Affiliations

Affiliations

  • 1 Department of Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, Shanghai, 200433, China.
  • 2 Department of Clinical Nutrition, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 201999, China.
  • 3 Department of Nutrition, The Third Affiliated Hospital of Naval Medical University, Shanghai, 200438, China.
  • 4 Department of Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, Shanghai, 200433, China. hongtao_lu1990@163.com.
  • 5 Department of Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, Shanghai, 200433, China. zqc417770627@live.com.
  • 6 Department of Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, Shanghai, 200433, China. shenhui_smmu@163.com.
  • # Contributed equally.
PMID: 38862478 DOI: 10.1038/s41420-024-02047-7
Abstract

Heat exposure is an environmental stressor that has been associated with cognitive impairment. However, the neural mechanisms that underlie this phenomenon have yet to be extensively investigated. The Morris water maze test was utilized to assess cognitive performance. RNA Sequencing was employed to discover the primary regulators and pathological pathways involved in cognitive impairment caused by heat. Before heat exposure in vivo and in vitro, activation of the sarco/endoplasmic reticulum (SR/ER) calcium (CA2+)-ATPase (SERCA) was achieved by CDN1163. Hematoxylin-Eosin, Nissl staining, calcium imaging, transmission electron microscopy, western blot, and immunofluorescence were utilized to visualize histological changes, intracellular calcium levels, endoplasmic reticulum stress (ERS) markers, Apoptosis, and synaptic proteins alterations. Heat stress (HS) significantly induced cognitive decline and neuronal damage in mice. By the transcriptome Sequencing between control (n = 5) and heat stress (n = 5) mice in hippocampal tissues, we identified a reduction in the expression of the atp2a gene encoding SERCA, accompanied by a corresponding decrease in its protein level. Consequently, this dysregulation resulted in an excessive accumulation of intracellular calcium ions. Furthermore, HS exposure also activated ERS and Apoptosis, as evidenced by the upregulation of p-PERK, p-eIF2α, CHOP, and Caspase-3. Consistently, a reduction in postsynaptic density protein 95 (PSD95) and synaptophysin (SYN) expressions indicated modifications in synaptic function. Notably, the impacts on neurons caused by HS were found to be mitigated by CDN1163 treatment both in vivo and in vitro. Additionally, SERCA-mediated ERS-induced Apoptosis was attenuated by GSK2606414 treatment via inhibiting PERK-eIF2α-CHOP axis that not only curtailed the level of Caspase-3 but also elevated the levels of PSD95 and SYN. These findings highlight the significant impact of heat stress on cognitive impairment, and further elucidate the underlying mechanism involving SERCA/PERK/eIF2α pathway.

Figures
Products