2. Academic Validation
  3. A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation

A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation

  • Nat Commun. 2024 Jun 14;15(1):5107. doi: 10.1038/s41467-024-49102-6.
Li Lin Xu 1 2,  Meng Qi Cui 1 2,  Chen Xu 1 2,  Miao Jing Zhang 1,  Gui Xin Li 3,  Ji Ming Xu 1,  Xiao Dan Wu 4,  Chuan Zao Mao 1,  Wo Na Ding 5,  Moussa Benhamed 6,  Zhong Jie Ding 7,  Shao Jian Zheng 8 9
Affiliations

Affiliations

  • 1 State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China.
  • 2 Guangdong Laboratory for Lingnan Modern Agriculture, College of Natural Resources and Environment, South China Agricultural University, 5100642, Guangzhou, China.
  • 3 College of Agronomy and Biotechnology, Zhejiang University, 310058, Hangzhou, China.
  • 4 Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, 310058, Hangzhou, China.
  • 5 Ningbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, 315300, Ningbo, China.
  • 6 Université Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 10 91405, Orsay, France.
  • 7 State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China. zjding@zju.edu.cn.
  • 8 State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China. sjzheng@zju.edu.cn.
  • 9 Guangdong Laboratory for Lingnan Modern Agriculture, College of Natural Resources and Environment, South China Agricultural University, 5100642, Guangzhou, China. sjzheng@zju.edu.cn.
PMID: 38877001 DOI: 10.1038/s41467-024-49102-6
Abstract

Inositol hexaphosphate (InsP6) is the major storage form of phosphorus in seeds. Reducing seed InsP6 content is a breeding objective in agriculture, as InsP6 negatively impacts animal nutrition and the environment. Nevertheless, how InsP6 accumulation is regulated remains largely unknown. Here, we identify a clade of receptor-like cytoplasmic kinases (RLCKs), named Inositol Polyphosphate-related Cytoplasmic Kinases 1-6 (IPCK1-IPCK6), deeply involved in InsP6 accumulation. The InsP6 concentration is dramatically reduced in seeds of ipck quadruple (T-4m/C-4m) and quintuple (C-5m) mutants, accompanied with the obviously increase of phosphate (Pi) concentration. The plasma membrane-localized IPCKs recruit IPK1 involved in InsP6 synthesis, and facilitate its binding and activity via phosphorylation of GRF 14-3-3 proteins. IPCKs also recruit IPK2s and PI-PLCs required for InsP4/InsP5 and InsP3 biosynthesis respectively, to form a potential IPCK-GRF-PLC-IPK2-IPK1 complex. Our findings therefore uncover a regulatory mechanism of InsP6 accumulation governed by IPCKs, shedding light on the mechanisms of InsP biosynthesis in eukaryotes.

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