TLR9-dependent dendritic cell maturation promotes IL-6-mediated upregulation of cathepsin X

Cysteine cathepsins are lysosomal proteases subject to dynamic regulation within antigen-presenting cells during the immune response and associated diseases. To investigate the regulation of Cathepsin X, a carboxy-mono-exopeptidase, during maturation of dendritic cells (DCs), we exposed immortalized mouse DCs to various Toll-like Receptor agonists. Using a Cathepsin X-selective activity-based probe, sCy5-Nle-SY, we observed a significant increase in Cathepsin X activation upon TLR-9 agonism with CpG, and to a lesser extent with Pam3 (TLR1/2), FSL-1 (TLR2/6) and LPS (TLR4). Despite clear maturation of DCs in response to Poly I:C (TLR3), Cathepsin X activity was only slightly increased by this agonist, suggesting differential regulation of Cathepsin X downstream of TLR activation. We demonstrated that Cathepsin X was upregulated at the transcriptional level in response to CpG. This occurred at late time points and was not dampened by NF-κB inhibition. Factors secreted from CpG-treated cells were able to provoke Cathepsin X upregulation when applied to naïve cells. Among these factors was IL-6, which on its own was sufficient to induce transcriptional upregulation and activation of Cathepsin X. IL-6 is highly secreted by DCs in response to CpG but much less so in response to poly I:C, and inhibition of the IL-6 receptor subunit glycoprotein 130 prevented CpG-mediated Cathepsin X upregulation. Collectively, these results demonstrate that Cathepsin X is differentially transcribed during DC maturation in response to diverse stimuli, and that secreted IL-6 is critical for its dynamic regulation.

IL‐6; Toll‐like receptors; cathepsin X; dendritic cells; protease regulation.