2. Academic Validation
  3. Acoustic-holography-patterned primary hepatocytes possess liver functions

Acoustic-holography-patterned primary hepatocytes possess liver functions

  • Biomaterials. 2024 Jul 1:311:122691. doi: 10.1016/j.biomaterials.2024.122691.
Changcan Li 1,  Gang Xu 2,  Yinhan Wang 3,  Laixin Huang 4,  Feiyan Cai 4,  Long Meng 4,  Bao Jin 3,  Zhuoran Jiang 5,  Hang Sun 3,  Haitao Zhao 3,  Xin Lu 3,  Xingting Sang 3,  Pengyu Huang 6,  Fei Li 7,  Huayu Yang 8,  Yilei Mao 9,  Hairong Zheng 10
Affiliations

Affiliations

  • 1 Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China; Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
  • 2 Liver Transplant Center, Organ Transplant Center, West China Hospital of Sichuan University, Chengdu, China.
  • 3 Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China.
  • 4 Shenzhen Institute of Advanced Technology, And Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, Shenzhen, China.
  • 5 Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK.
  • 6 Institute of Biomedical Engineering, PUMC & Chinese Academy of Medical Sciences (CAMS), Tianjin, China.
  • 7 Shenzhen Institute of Advanced Technology, And Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, Shenzhen, China. Electronic address: fei.li@siat.ac.cn.
  • 8 Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China. Electronic address: yanghuayu@pumch.cn.
  • 9 Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China. Electronic address: maoyilei@pumch.cn.
  • 10 Shenzhen Institute of Advanced Technology, And Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, Shenzhen, China. Electronic address: hr.zheng@siat.ac.cn.
PMID: 38996673 DOI: 10.1016/j.biomaterials.2024.122691
Abstract

Acoustic holography (AH), a promising approach for cell patterning, emerges as a powerful tool for constructing novel invitro 3D models that mimic organs and cancers features. However, understanding changes in cell function post-AH remains limited. Furthermore, replicating complex physiological and pathological processes solely with cell lines proves challenging. Here, we employed acoustical holographic lattice to assemble primary hepatocytes directly isolated from mice into a cell cluster matrix to construct a liver-shaped tissue sample. For the first time, we evaluated the liver functions of AH-patterned primary hepatocytes. The patterned model exhibited large numbers of self-assembled spheroids and superior multifarious core hepatocyte functions compared to cells in 2D and traditional 3D culture models. AH offers a robust protocol for long-term in vitro culture of primary cells, underscoring its potential for future applications in disease pathogenesis research, drug testing, and organ replacement therapy.

Keywords

3D model; Acoustic holography; Liver function; Primary hepatocytes.

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