2. Academic Validation
  3. MitoAMPK inhibits the Warburg effect by MZF1-SIRT6 with glycosis related genes in NSCLC

MitoAMPK inhibits the Warburg effect by MZF1-SIRT6 with glycosis related genes in NSCLC

  • J Cell Mol Med. 2024 Sep;28(17):e70053. doi: 10.1111/jcmm.70053.
Shangyu Li 1,  Jinyao He 1 2,  Lijie Zhang 1,  Qiaojiajie Zhao 1,  Shuqi Zhao 1,  Shanshan Jiang 1
Affiliations

Affiliations

  • 1 Institute of Hematological Research, Shaanxi Provincial People's Hospital, Xi'an, China.
  • 2 Department of Clinical Laboratory Diagnostics, Xi'an Medical University, Xi'an, China.
PMID: 39224032 DOI: 10.1111/jcmm.70053
Abstract

MitoAMPK was proved to inhibit the Warburg effect, but the specific mechanisms on non-small-cell lung Cancer remain unclear. Here, we selected SIRT6 and MZF1 to clarify the mechanism. By western blotting, quantitative polymerase chain reaction, the CCK-8 assay, and immunohistochemistry assays, we found SIRT6 expression was lower in NSCLC tissues and cell lines than normal tissues and cells. Moreover, SIRT6 could inhibit the Warburg effect by regulating glycolysis-related genes of SLC2A2, SLC2A4 and PKM2. Finally, we demonstrated the interaction between SIRT6 and MZF1 using ChIP-qPCR. In conclusion, mitoAMPK inhibits the Warburg effect by regulating the expression of the MZF1-SIRT6 complex.

Keywords

MZF1; SIRT6; Warburg effect; glycolysis; mitochondrial AMPK; non‐small‐cell lung cancer.

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