2. Academic Validation
  3. Salvianolic acid A inhibits pseudorabies virus infection by directly inactivating the virus particle

Salvianolic acid A inhibits pseudorabies virus infection by directly inactivating the virus particle

  • Phytomedicine. 2024 Nov:134:156015. doi: 10.1016/j.phymed.2024.156015.
Zilu Chen 1,  Dongliang Li 2,  Tianliang Wang 1,  Yaqin Li 1,  Panpan Qin 1,  Hongsen Zhu 3,  Mengjia Zhang 4,  Wentao Li 5,  Linyang Yu 2,  Hong Duan 2,  Lu Chen 2,  Yongtao Li 6,  Guanmin Zheng 7
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, PR China.
  • 2 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, PR China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan, Agricultural University, Zhengzhou 450046, PR China.
  • 3 School of Medicine, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.
  • 4 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China.
  • 5 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, 430070, PR China.
  • 6 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, PR China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan, Agricultural University, Zhengzhou 450046, PR China. Electronic address: yongtaole@126.com.
  • 7 School of Medicine, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China. Electronic address: guanminzheng308@163.com.
PMID: 39244942 DOI: 10.1016/j.phymed.2024.156015
Abstract

Background: Pseudorabies virus (PRV), a member of the family Herpesviridae, is responsible for significant economic losses in the pig industry and has recently been associated with human viral encephalitis, leading to severe neurological symptoms post-recovery. Despite the widespread impact of PRV, there are currently no approved effective drugs for treating PRV-related diseases in humans or pigs. Therefore, the exploration and discovery of safe and effective drugs for the prevention and treatment of PRV Infection is of paramount importance.

Purpose: The objective of this study is to screen and identify natural compounds with Antiviral activity against PRV.

Methods: First, we used a strain of PRV with green Fluorescent protein (PRV-GFP) to screen a natural product chemical library to identify potential Antiviral drugs. Next, we assessed the Antiviral abilities of salvianolic acid A (SAA) in vitro using virus titer assay, qPCR, and IFA. We investigated the mechanisms of SAA's Antiviral activity through viral attachment, internalization, inactivation, and Nuclease digestion assay. Finally, we evaluated the efficacy of SAA in inactivating PRV using mice as the experimental subjects.

Results: This study screened 206 natural compounds for anti-PRV activity in vitro, resulting in the identification of seven potential Antiviral agents. Notably, SAA emerged as a promising candidate with significant anti-PRV activity. The mechanism of action may be that SAA can directly inactivate the virus by disrupting viral envelope. In vivo experiments have shown that pre-incubation of SAA and PRV can effectively inhibit the infectivity and pathogenicity of PRV in mice.

Conclusion: This study offers valuable insights into the Antiviral properties of SAA, potentially informing strategies for controlling PRV epidemics and treating related diseases in both humans and Animals.

Keywords

Antiviral; Envelope; Inactivation; Pseudorabies virus; Salvianolic acid A.

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