2. Academic Validation
  3. Protocol for studying macrophage lipid crosstalk with murine tumor cells

Protocol for studying macrophage lipid crosstalk with murine tumor cells

  • STAR Protoc. 2024 Dec 20;5(4):103421. doi: 10.1016/j.xpro.2024.103421.
Daan J Kloosterman 1,  Martina Farber 2,  Menno Boon 2,  Johanna Erbani 3,  Leila Akkari 4
Affiliations

Affiliations

  • 1 Division of Tumour Biology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, the Netherlands. Electronic address: d.kloosterman@nki.nl.
  • 2 Division of Tumour Biology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, the Netherlands.
  • 3 Division of Tumour Biology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, the Netherlands. Electronic address: j.erbani@nki.nl.
  • 4 Division of Tumour Biology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, the Netherlands. Electronic address: l.akkari@nki.nl.
PMID: 39488834 DOI: 10.1016/j.xpro.2024.103421
Abstract

Lipid accumulation has recently emerged as a key feature underlying the pro-tumorigenic role of macrophages. Here, we present a workflow to study macrophage lipid crosstalk with tumor cells. We describe steps for the identification, purification, and multi-omics characterization of lipid-laden macrophages (LLMs) from murine tumors and outline protocols to assess the functional significance of LLMs in Cancer malignancy. This approach has the potential to uncover the source of lipids that drives LLM formation and its pro-tumorigenic potential in multiple Cancer types. For complete details on the use and execution of this protocol, please refer to Kloosterman, Erbani, et al.1.

Keywords

RNA-seq; bioinformatics; cancer; cell biology; flow cytometry; genomics; immunology; microbiology; sequence analysis; single cell.

Figures
Products