2. Academic Validation
  3. HFM1 is essential for the germ cell intercellular bridge transport in primordial follicle formation in mice

HFM1 is essential for the germ cell intercellular bridge transport in primordial follicle formation in mice

  • Cell Mol Life Sci. 2024 Dec 27;82(1):28. doi: 10.1007/s00018-024-05541-4.
Yuheng He # 1,  Huiyuan Wang # 1,  Tongtong Hong 1,  Luanqian Hu 1,  Chao Gao 1,  Li Gao 1,  Yugui Cui 1,  Rongrong Tan 2,  Danhua Pu 3,  Jie Wu 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital/Jiangsu Women and Children Health Hospital, Nanjing, 210036, China.
  • 2 State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital/Jiangsu Women and Children Health Hospital, Nanjing, 210036, China. tanrongrong86119@163.com.
  • 3 State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital/Jiangsu Women and Children Health Hospital, Nanjing, 210036, China. pudanhua@139.com.
  • 4 State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital/Jiangsu Women and Children Health Hospital, Nanjing, 210036, China. wujiemd@126.com.
  • # Contributed equally.
PMID: 39725823 DOI: 10.1007/s00018-024-05541-4
Abstract

The reproductive lifespan of female mammals is determined by the size of the primordial follicle pool, which comprises oocytes enclosed by a layer of flattened pre-granulosa cells. Oocyte differentiation needs acquiring organelles and cytoplasm from sister germ cells in cysts, but the mechanisms regulating this process remain unknown. Previously helicase for meiosis 1 (HFM1) is reported to be related to the development of premature ovarian insufficiency. Here, it is found that HFM1 is involved in oocyte differentiation through organelle enrichment from sister germ cells. Further study indicates that HFM1 is involved in intercellular directional transport through intercellular bridges via the RAC1/ANLN/E-cad signaling pathway, which is indispensable for oocyte differentiation and primordial follicle formation. These findings shed light on the critical role of HFM1 in intercellular bridge transport, which is essential for the establishment of the primordial follicle pool and presenting new horizons for female fertility protection.

Keywords

HFM1; Intercellular bridge; Oocyte differentiation; POI; Primordial follicle formation.

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