2. Academic Validation
  3. Viral Mimetic Bacterial Outer Membrane Vesicles for Targeting Angiotensin-Converting Enzyme 2

Viral Mimetic Bacterial Outer Membrane Vesicles for Targeting Angiotensin-Converting Enzyme 2

  • Int J Nanomedicine. 2025 Jan 16:20:669-684. doi: 10.2147/IJN.S497742.
Gna Ahn # 1 2,  Hyo-Won Yoon # 1,  Ju Hwan Jeong # 3 4,  Yang-Hoon Kim # 1,  Woo-Ri Shin 1 5 6,  Min-Suk Song 3,  Ji-Young Ahn 1
Affiliations

Affiliations

  • 1 Department of Microbiology, Chungbuk National University, Cheongju, Republic of Korea.
  • 2 Center for Ecology and Environmental Toxicology, Chungbuk National University, Cheongju, Republic of Korea.
  • 3 Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • 4 Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea.
  • 5 Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
  • 6 Department of Animal Bioscience & Integrated Biotechnology, Gyeongsang National University, Jinju, Republic of Korea.
  • # Contributed equally.
PMID: 39835181 DOI: 10.2147/IJN.S497742
Abstract

Purpose: Outer membrane vesicles (OMVs) derived from Gram-negative bacteria naturally serve as a heterologous nano-engineering platform, functioning as effective multi-use nanovesicles for diagnostics, vaccines, and treatments against pathogens. To apply refined OMVs for human theranostic applications, we developed naturally exposed receptor-binding domain (RBD) OMVs grafted with antigen 43 as a minimal modular system targeting angiotensin-converting Enzyme 2 (ACE2).

Methods: We constructed E. coli-derived OMVs using the antigen 43 autotransporter system to display RBD referred to as viral mimetic Ag43β700_RBD OMVs. Based on this, Ag43β700_RBD protein were expressed onto Escherichia coli (E. coli) membrane. Artificial viral mimetic Ag43β700_RBD OMVs were fabricated by self-assembly through membrane disruption of the Ag43β700_RBD E. coli using a chemical detergent mainly containing lysozyme. Through serial centrifugation to purify fabricated OMVs, spherical Ag43β700_RBD OMVs with an average diameter of 218 nm were obtained. The confirmation of the RBD expressed on OMVs was performed using trypsin treatment.

Results: Our viral mimetic Ag43β700_RBD OMVs had an impact on the theranostic studies: (i) angiotensin-converting Enzyme 2 blockade assay, (ii) enzyme-linked immunosorbent assay for the OMVs, and (iii) intracellular uptake and neutralization assay. As serodiagnostic surrogates, Ag43β700_RBD OMVs were applied to ACE2 blockade and OMVs-ELISA assay to quantify neutralization Antibodies (nAbs). They reduced the robust immune response in vitro, especially IL-6 and IL-1β. Experiments in mice, Ag43β700_RBD OMVs was successfully proven to be safe and effective; they produced a detectable level of nAbs with 39-58% neutralisation and reduced viral titres in the lungs and brain without weight loss.

Conclusion: The developed viral mimetic Ag43β700_RBD OMVs may therefore be applied as a nanovesicle-theranostic platform for further emerging infectious disease-related diagnosis, vaccination, and treatment.

Keywords

angiotensin-converting enzyme 2; antigen 43 autotransporters; outer membrane vesicle; targeted delivery vehicle; theranostics.

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