2. Academic Validation
  3. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes

Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes

  • Anal Biochem. 1984 Nov 15;143(1):30-4. doi: 10.1016/0003-2697(84)90553-0.
S S Twining
PMID: 6442109 DOI: 10.1016/0003-2697(84)90553-0
Abstract

A simple inexpensive sensitive Protease assay was developed using soluble fluorescein isothiocyanate (FITC)-labeled casein. Casein was reacted with FITC to form the fluorescein thiocarbamoyl derivative. This substrate is cleaved by trypsin, chymotrypsin, Elastase, subtilisin, and thermolysin in a linear time-dependent manner. These Enzymes can be measured in the nanogram and subnanogram range using this assay. The assay is reproducible, has a low blank, and uses casein, which resembles natural substrates of most proteases.

Figures
Products