2. Academic Validation
  3. Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function

Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function

  • Nature. 1994 Oct 27;371(6500):762-7. doi: 10.1038/371762a0.
A Pause 1,  G J Belsham,  A C Gingras,  O Donzé,  T A Lin,  J C Lawrence Jr,  N Sonenberg
Affiliations

Affiliation

  • 1 Department of Biochemistry, McGill University, Montréal, Québec, Canada.
PMID: 7935836 DOI: 10.1038/371762a0
Abstract

The cloning is described of two related human complementary DNAs encoding polypeptides that interact specifically with the translation initiation factor eIF-4E, which binds to the messenger RNA 5'-cap structure. Interaction of these proteins with eIF-4E inhibits translation but treatment of cells with Insulin causes one of them to become hyperphosphorylated and dissociate from eIF-4E, thereby relieving the translational inhibition. The action of this new regulator of protein synthesis is therefore modulated by Insulin, which acts to stimulate the overall rate of translation and promote cell growth.

Figures