N3-Cho (Synonyms: Azido-choline)

Cat. No.: HY-W588725: FAQs
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N3-Cho (Azido-choline) is a click chemistry reagent containing an azide group that can be used in the synthesis of cell membrane structures.

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N3-Cho Chemical Structure

CAS No. : 1239916-55-2

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Description

N3-Cho (Azido-choline) is a click chemistry reagent containing an azide group that can be used in the synthesis of cell membrane structures^[1].

Molecular Weight

159.21

Formula

C₆H₁₅N₄O⁺

CAS No.

1239916-55-2

SMILES

OCC[N+](C)(CCN=[N+]=[N-])C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (785.13 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.2810 mL	31.4051 mL	62.8101 mL
5 mM	1.2562 mL	6.2810 mL	12.5620 mL
10 mM	0.6281 mL	3.1405 mL	6.2810 mL

View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Masaki Tsuchiya, et al. Organelle-selective click labeling coupled with flow cytometry allows pooled CRISPR screening of genes involved in phosphatidylcholine metabolism. Cell Metab. 2023 Jun 6;35(6):1072-1083.e9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	6.2810 mL	31.4051 mL	62.8101 mL	157.0253 mL
	5 mM	1.2562 mL	6.2810 mL	12.5620 mL	31.4051 mL
	10 mM	0.6281 mL	3.1405 mL	6.2810 mL	15.7025 mL
	15 mM	0.4187 mL	2.0937 mL	4.1873 mL	10.4684 mL
	20 mM	0.3141 mL	1.5703 mL	3.1405 mL	7.8513 mL
	25 mM	0.2512 mL	1.2562 mL	2.5124 mL	6.2810 mL
	30 mM	0.2094 mL	1.0468 mL	2.0937 mL	5.2342 mL
	40 mM	0.1570 mL	0.7851 mL	1.5703 mL	3.9256 mL
	50 mM	0.1256 mL	0.6281 mL	1.2562 mL	3.1405 mL
	60 mM	0.1047 mL	0.5234 mL	1.0468 mL	2.6171 mL
	80 mM	0.0785 mL	0.3926 mL	0.7851 mL	1.9628 mL
	100 mM	0.0628 mL	0.3141 mL	0.6281 mL	1.5703 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N3-Cho1239916-55-2Azido-cholineOthersclick chemistry reagentazide groupmembrane structuresInhibitorinhibitorinhibit

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