2. NF-κB Metabolic Enzyme/Protease
  3. Keap1-Nrf2 Endogenous Metabolite
  4. PRL-295

PRL-295 is an orally active inhibitor targeting Keap1-Nrf2 interaction。PRL-295 increases the thermal stability of Keap1 and disrupts its interaction with Nrf2, thereby activating the Nrf2-dependent transcriptional target NAD(P)H:quinone oxidoreductase 1 (NQO1). PRL-295 protects against Acetaminophen (HY-66005)-induced liver injury in mice.

For research use only. We do not sell to patients.

PRL-295 Chemical Structure

PRL-295 Chemical Structure

CAS No. : 2377770-85-7

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

PRL-295 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PRL-295 is an orally active inhibitor targeting Keap1-Nrf2 interaction。PRL-295 increases the thermal stability of Keap1 and disrupts its interaction with Nrf2, thereby activating the Nrf2-dependent transcriptional target NAD(P)H:quinone oxidoreductase 1 (NQO1). PRL-295 protects against Acetaminophen (HY-66005)-induced liver injury in mice[1].

In Vitro

PRL-295 increases the thermostability of Keap1 in Keap1-mCherry-expressing U2OS cells lysates (15 μM, 1 h), HL-60 cells lysates (30 μM, 1 h) and HL-60 cells (10 μM, 3 h)[1].
PRL-295 (50 μM, 1 h) disrupts the Keap1-Nrf2 protein complex in single HeLa cell co-expressing sfGFP-Nrf2 and Keap1-mCherry[1].
PRL-295 (approximately 60 nM-10 μM, 48 h) concentration-dependently induces the Nrf2 target NAD(P)H:quinone oxidoreductase 1 (NQO1) in Hepa1c1c7 and ARPE-19 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PRL-295 Related Antibodies
In Vivo

PRL-295 (10-50 mg/kg, p.o., 4 times, 24 h apart) dose-dependently increases the thermal stability of Keap1 and activates the Nrf2 transcriptional target NQO1 in murine liver[1].
PRL-295 (25 mg/kg, p.o., 3 days) protects against Acetaminophen (HY-66005)-induced liver injury in C57/BL6 mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 8-week-old male C57/BL6 mice were intraperitoneally Acetaminophen (HY-66005) (300 mg/kg)[1]
Dosage: 25 mg/kg
Administration: Oral gavage (p.o.); once a day, over a period of 3 days
Result: Reduced plasma levels of alanine aminotransferase and glutamine aminotransferase.
Animal Model: Male C57BL/6 wild-type mice[1]
Dosage: 10, 25, 50 mg/kg
Administration: Oral gavage (p.o.); 4 times, 24 h apart
Result: Increased hepatic NQO1 mRNA 2.2-fold or 2.8-fold in a dose-dependent manner at 10 mg/kg or 25 mg/kg, respectively.
Increased NQO1 mRNA moderately (1.5-fold) in kidneys at a dose of 25 mg/kg.
Did not significantly increase mRNA levels of NQO1 in the brain, colon, or lung.
Molecular Weight

639.62

Formula

C27H24F3N3O8S2
CAS No.
SMILES

O=C(CN(S(=O)(C1=CC=C(C=C1)OC)=O)C2=NC=C(N(S(=O)(C3=CC=C(C=C3)OC)=O)CC(F)(F)F)C4=C2C=CC=C4)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

PRL-295 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PRL-2952377770-85-7PRL295PRL 295Keap1-Nrf2Endogenous MetaboliteThermostabilityU2OSHeLaHL-60HepatotoxicityC57BL/6JInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PRL-295
Cat. No.:
HY-171035
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (0 KB) Français - FR (0 KB) Deutsch - DE (0 KB) Norwegian - NO (0 KB) Español - ES (0 KB) Swedish - SV (0 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.