2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. Recombinant Proteinase K

Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations.

For research use only. We do not sell to patients.

Recombinant Proteinase K Chemical Structure

Recombinant Proteinase K Chemical Structure

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Recombinant Proteinase K Related Antibodies

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Description

Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations[1].

In Vitro

Proteinase K digestion[1]
1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.
2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 μl aliquot to get the final concentrations of 2, 4 and 8 μg/ml. Reactions are carried out at 37°C for 30 minutes.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Recombinant Proteinase K Related Antibodies
Appearance

Solid

Color

Off-white to light yellow

SMILES

[Recombinant Proteinase K]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

Purity: ≥95.0%

SDS (251 KB)

COA (241 KB)

Handling Instructions (2659 KB)
References
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Recombinant Proteinase K Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Recombinant Proteinase KSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesaromatic amino acidcarboxy-terminated peptide nucleic aciddigestionInhibitorinhibitorinhibit

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