2B4/CD244 Protein, Human (HEK293, Fc)

2B4/CD244 protein exhibits heightened affinity for binding to CD48, surpassing isoform 1, leading to increased cytotoxicity and intracellular calcium release. The stronger interaction implies more robust engagement, potentially amplifying cellular responses, particularly heightened cytotoxic activity and intracellular calcium release. 2B4/CD244 Protein, Human (200a.a, HEK293, Fc) is the recombinant human-derived 2B4/CD244 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of 2B4/CD244 Protein, Human (200a.a, HEK293, Fc) is 200 a.a., with molecular weight of 49 kDa.

The 2B4/CD244 protein demonstrates a higher affinity for binding to CD48 compared to isoform 1, and this interaction results in heightened cytotoxicity and intracellular calcium release. The stronger binding between 2B4/CD244 and CD48 suggests a more robust engagement, potentially leading to enhanced cellular responses, including increased cytotoxic activity and release of intracellular calcium.

Immobilized Human CD48, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC₅₀ of ≤0.45 μg/mL determined by ELISA.

Human

HEK293

C-hFc

Q9BZW8-2 (C22-R221)

51744  [NCBI]

68-78 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.