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  4. Aconitase 2/ACO2 Protein, Human (sf9, His-GST)

Aconitase 2/ACO2 Protein, Human (sf9, His-GST)

Cat. No.: HY-P74432
SDS COA Handling Instructions

Aconitase 2/ACO2 Protein, an enzyme, plays a vital role in the tricarboxylic acid cycle and energy production. Dysregulation of Aconitase 2/ACO2 Protein has been associated with mitochondrial dysfunction and oxidative stress. Targeting Aconitase 2/ACO2 Protein may provide potential therapeutic strategies by regulating energy metabolism, reducing oxidative damage, and improving mitochondrial function. Aconitase 2/ACO2 Protein, Human (sf9, His-GST) is the recombinant human-derived Aconitase 2/ACO2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Aconitase 2/ACO2 Protein, Human (sf9, His-GST) is 753 a.a., with molecular weight of ~110 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $70 In-stock
10 μg $115 In-stock
50 μg $300 In-stock
100 μg $510 In-stock
> 100 μg   Get quote  

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Description

Aconitase 2/ACO2 Protein, an enzyme, plays a vital role in the tricarboxylic acid cycle and energy production. Dysregulation of Aconitase 2/ACO2 Protein has been associated with mitochondrial dysfunction and oxidative stress. Targeting Aconitase 2/ACO2 Protein may provide potential therapeutic strategies by regulating energy metabolism, reducing oxidative damage, and improving mitochondrial function. Aconitase 2/ACO2 Protein, Human (sf9, His-GST) is the recombinant human-derived Aconitase 2/ACO2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Aconitase 2/ACO2 Protein, Human (sf9, His-GST) is 753 a.a., with molecular weight of ~110 kDa.

Background

The Aconitase 2/ACO2 protein emerges as a pivotal catalyst in cellular processes, facilitating the isomerization of citrate to isocitrate through the intermediate cis-aconitate. This enzymatic activity is instrumental in key metabolic pathways, contributing to the dynamic regulation of citric acid cycle intermediates. The ACO2 protein's role in orchestrating the conversion of citrate to isocitrate underscores its significance in maintaining cellular energy balance and metabolic homeostasis.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

Q99798 (Q28-Q780)

Gene ID

50  [NCBI]

Molecular Construction
N-term
His-GST
ACO2 (Q28-Q780)
Accession # Q99798
C-term
Synonyms
Aconitate hydratase; Aconitase; ACO2
Molecular Weight

Approximately 110 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, 0.5 mM TCEP, pH 8.0, 0.5 mM GSH.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Aconitase 2/ACO2 Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Aconitase 2/ACO2 Protein, Human (sf9, His-GST)
Cat. No.:
HY-P74432
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