ACOX1 Protein, Human (sf9, His)

The ACOX1 protein is critical in the initial steps of peroxisomal β-oxidation, especially of very long-chain fatty acids. It catalyzes the desaturation of fatty acyl-CoA (such as palmitoyl-CoA) to 2-trans-enoyl-CoA. ACOX1 Protein, Human (sf9, His) is the recombinant human-derived ACOX1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ACOX1 Protein, Human (sf9, His) is 660 a.a., with molecular weight of ~60 kDa.

The ACOX1 Protein plays a pivotal role in the initial and rate-limiting step of peroxisomal beta-oxidation, particularly of straight-chain saturated and unsaturated very-long-chain fatty acids. Its catalytic activity involves the desaturation of fatty acyl-CoAs, such as palmitoyl-CoA, into 2-trans-enoyl-CoAs, exemplified by (2E)-hexadecenoyl-CoA. Additionally, ACOX1 contributes to the production of hydrogen peroxide (H(2)O(2)) by donating electrons directly to molecular oxygen (O(2)). While displaying notable activity against medium-chain fatty acyl-CoAs, its optimum activity is observed with a chain length of 10 carbons, exemplified by decanoyl-CoA, as demonstrated in vitro. This multifaceted enzymatic activity underscores ACOX1's integral role in peroxisomal beta-oxidation and the metabolism of various fatty acid substrates.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

AAH08767.1 (M1-L660)

51  [NCBI]

Approximately 60 kDa

Greater than 85% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.0, 20% gly, 3 mM DTT.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.