ADH4 Protein, Saccharomyces cerevisiae (His, Strep)

The ADH4 protein is a specific alcohol dehydrogenase that primarily functions as a mitochondrial formaldehyde dehydrogenase with a unique ethanol preference. It does not affect ethanol production and exhibits reduced activity on primary alcohols with four or more carbon atoms. ADH4 Protein, Saccharomyces cerevisiae (His, Strep) is the recombinant ADH4 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of ADH4 Protein, Saccharomyces cerevisiae (His, Strep) is 381 a.a., .

ADH4 Protein functions as a specific alcohol dehydrogenase with a distinct preference for ethanol, primarily serving as a mitochondrial formaldehyde dehydrogenase without influencing ethanol production. Its enzymatic activity is notably diminished towards primary alcohols containing four carbon atoms or more, with isomers of aliphatic alcohol, secondary alcohols, and glycerol exhibiting no reactivity. While the precise role of ADH4 in yeast metabolism remains unclear, it is established that ADH4 does not play a role in ethanol production during growth on glucose, nor is it involved in the oxidation of ethanol to acetaldehyde. This delineation of substrate specificity and functional characteristics provides insights into ADH4's specific role within cellular processes, shedding light on its unique contribution to alcohol metabolism and mitochondrial formaldehyde oxidation.

Others

E. coli

N-Strep;N-6*His

P10127 (S2-Y382)

852636

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.