ADH5 Protein, Human

The ADH5 protein catalyzes the oxidation of long-chain primary alcohols and S-(hydroxymethyl) glutathione. It also oxidizes long-chain omega-hydroxy fatty acids, producing intermediate aldehydes and end products. However, it is ineffective in oxidizing ethanol. ADH5 is crucial for clearing cytotoxic formaldehyde, which causes DNA damage. ADH5 Protein, Human is the recombinant human-derived ADH5 protein, expressed by E. coli , with no tag.

ADH5, a Class-III alcohol dehydrogenase, plays a pivotal role in cellular detoxification processes by catalyzing the oxidation of long-chain primary alcohols and S-(hydroxymethyl) glutathione. Additionally, ADH5 is involved in the oxidation of long-chain omega-hydroxy fatty acids, such as 20-hydroxyeicosatetraenoic acid (20-HETE), yielding both the intermediate aldehyde, 20-oxoarachidonate, and the final product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Despite its classification as an alcohol dehydrogenase, ADH5 exhibits low efficiency in oxidizing ethanol. Notably, ADH5 is crucial for cellular defense against formaldehyde, a cytotoxic and carcinogenic metabolite, contributing to the clearance of this substance and preventing potential DNA damage.

Human

E. coli

Tag Free

P11766 (M1-I374)

128

39.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.