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  3. Allergen Fel d 4 Protein, Cat (His-SUMO)

Allergen Fel d 4 Protein, Cat (His-SUMO)

Cat. No.: HY-P71573
Handling Instructions

Allergen Fel d 4 Protein, Cat (His-SUMO) is a recombinant protein with a His-SUMO tag. Fel d 4 is a lipocalin allergen produced by the cat, which binds IgE at relatively high frequency in cat-sensitive individuals.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Allergen Fel d 4 Protein, Cat (His-SUMO) is a recombinant protein with a His-SUMO tag. Fel d 4 is a lipocalin allergen produced by the cat, which binds IgE at relatively high frequency in cat-sensitive individuals[1].

Background

Fel d 4 is a lipocalin allergen produced by the cat, which binds IgE at relatively high frequency in cat-sensitive individuals. The allergen provides not only a means for investigating differences in the immune response to lipocalin allergens from that found for other mammalian species but also an important reagent for the diagnosis of cat allergy[1].

Species

Cat

Source

E. coli

Tag

N-His;N-SUMO

Accession

Q5VFH6 (H16-E186)

Gene ID

493739  [NCBI]

Molecular Construction
N-term
6*His-SUMO
Feld4 (H16-E186)
Accession # Q5VFH6
C-term
Synonyms
Allergen Fel d 4; allergen Fel d 4
AA Sequence

HEEENVVRSNIDISKISGEWYSILLASDVKEKIEENGSMRVFVEHIKALDNSSLSFVFHTKENGKCTEIFLVADKTKDGVYTVVYDGYNVFSIVETVYDEYILLHLLNFDKTRPFQLVEFYAREPDVSQKLKEKFVKYCQEHGIVNILDLTEVDRCLQARGSEVAQDSSVE

Molecular Weight

Approximately 35.7 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
References

Allergen Fel d 4 Protein, Cat (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Allergen Fel d 4 Protein, Cat (His-SUMO)
Cat. No.:
HY-P71573
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