Animal-Free HDGF Protein, Human (His)

The HDGF protein acts as a transcriptional repressor and was previously thought to have mitogenic activity for fibroblasts and be a heparin-binding protein. However, recent studies have shown that it does not exhibit mitogenic activity for fibroblasts and does not bind heparin. Animal-Free HDGF Protein, Human (His) is the recombinant human-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag.

Hepatoma-derived growth factor (HDGF) exhibits a dual role as both a transcriptional repressor and a mitogenic factor for fibroblasts. While it acts as a repressor in transcriptional regulation, influencing cellular processes, it also demonstrates mitogenic activity, particularly for fibroblasts. HDGF has been identified as a heparin-binding protein, which suggests its involvement in interactions with heparin and potentially modulating cellular responses. However, it's noteworthy that conflicting reports exist, with some studies indicating that HDGF lacks mitogenic activity for fibroblasts and does not bind to heparin. These findings highlight the complexity of HDGF's functional repertoire, showcasing its diverse roles in transcriptional control, mitogenic signaling, and potential interactions with heparin.

Human

E. coli

C-His

P51858-1 (M1-L240)

3068  [NCBI]

MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

Approximately 29.60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.