2. Recombinant Proteins
  3. Cytokines and Growth Factors Animal-free Recombinant Proteins
  4. Animal-Free Laminin 521 Protein, Human

Animal-Free Laminin 521 Protein, Human

Cat. No.: HY-P701311AF
COA Handling Instructions

Laminins are a major component of basement membranes. Laminin 521 is a heterotrimeric glycoprotein composed of one α5, one β2, and one γ1 chain, is a defined matrix for feeder-free culture of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human ESCs and iPSCs without spontaneous differentiation.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
100 μg $170 In-stock
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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Laminins are a major component of basement membranes. Laminin 521 is a heterotrimeric glycoprotein composed of one α5, one β2, and one γ1 chain, is a defined matrix for feeder-free culture of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human ESCs and iPSCs without spontaneous differentiation.
Species

Human
Source

HEK293
Accession

/
Gene ID

/
Synonyms
Laminin 521
Molecular Weight

90-110 kDa, 40-50 kDa, 27- 30 kDa
Purity

Greater than 95% as determined by SEC-HPLC.

Appearance

Solution
Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4 or PBS,10% Glycerol,pH7.4.
Endotoxin Level

<0.01 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (205 KB)

Handling Instructions (2659 KB)

Animal-Free Laminin 521 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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