Annexin A6/ANXA6 Protein, Human (His)

Annexin A6 Protein, Human (His) is a Annexin A6 protein fused to His-tag at N-terminus. Annexin A6 is an endogenous and secretory multifunctional protein and locates on the cell surface. Annexin A6 can be used for the study in mediating the endosome aggregation and vesicle fusion during exocytosis^[1][2].

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins.
Annexin 6 is a 67kDa calcium-dependent protein, a prime target for facilitating membrane coalescence and folding required during membrane repair. Annexin 6 is involved in the regulation of the release of Ca²⁺ from intracellular stores and may be associated with CD21. In addition, Annexin 6 has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

Human

E. coli

C-6*His

P08133 (A2-D673)

309  [NCBI]

AKPAQGAKYRGSIHDFPGFDPNQDAEALYTAMKGFGSDKEAILDIITSRSNRQRQEVCQSYKSLYGKDLIADLKYELTGKFERLIVGLMRPPAYCDAKEIKDAISGIGTDEKCLIEILASRTNEQMHQLVAAYKDAYERDLEADIIGDTSGHFQKMLVVLLQGTREEDDVVSEDLVQQDVQDLYEAGELKWGTDEAQFIYILGNRSKQHLRLVFDEYLKTTGKPIEASIRGELSGDFEKLMLAVVKCIRSTPEYFAERLFKAMKGLGTRDNTLIRIMVSRSELDMLDIREIFRTKYEKSLYSMIKNDTSGEYKKTLLKLSGGDDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED

Approximately 60-90 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Alexis R Demonbreun, et al. Recombinant annexin A6 promotes membrane repair and protects against muscle injury. J Clin Invest. 2019 Nov 1;129(11):4657-4670.  [Content Brief]

  [2]. Ramin Raouf, et al. Inhibition of somatosensory mechanotransduction by annexin A6. Sci Signal. 2018 Jun 19;11(535):eaao2060.  [Content Brief]

  [3]. Julie Leca, et al. Cancer-associated fibroblast-derived annexin A6+ extracellular vesicles support pancreatic cancer aggressiveness. J Clin Invest. 2016 Nov 1;126(11):4140-4156.  [Content Brief]