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Annexin A4/ANXA4 Protein, Mouse (His-Myc)

Cat. No.: HY-P72079
SDS COA Handling Instructions

The Annexin A4/ANXA4 protein is a calcium/phospholipid-binding protein that plays a key role in membrane dynamics by promoting membrane fusion and exocytosis. This protein exhibits specific affinity for calcium ions and phospholipids, allowing it to participate in cellular processes involving membrane interactions. Annexin A4/ANXA4 Protein, Mouse (His-Myc) is the recombinant mouse-derived Annexin A4/ANXA4 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of Annexin A4/ANXA4 Protein, Mouse (His-Myc) is 318 a.a., with molecular weight of ~40.8 kDa.

For research use only. We do not sell to patients.

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Description

The Annexin A4/ANXA4 protein is a calcium/phospholipid-binding protein that plays a key role in membrane dynamics by promoting membrane fusion and exocytosis. This protein exhibits specific affinity for calcium ions and phospholipids, allowing it to participate in cellular processes involving membrane interactions. Annexin A4/ANXA4 Protein, Mouse (His-Myc) is the recombinant mouse-derived Annexin A4/ANXA4 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of Annexin A4/ANXA4 Protein, Mouse (His-Myc) is 318 a.a., with molecular weight of ~40.8 kDa.

Background

Annexin A4 (ANXA4) is a calcium/phospholipid-binding protein known for its crucial role in membrane fusion and participation in exocytosis. Functioning as a mediator between calcium signaling and membrane dynamics, ANXA4 contributes to cellular processes involved in the release of cellular contents. Its ability to bind to both calcium ions and phospholipids suggests a regulatory role in membrane-related events, particularly those associated with vesicle trafficking and exocytosis. It has to highlight ANXA4's significance in calcium-dependent cellular mechanisms, emphasizing its involvement in promoting membrane fusion and facilitating exocytotic processes.

Species

Mouse

Source

E. coli

Tag

N-10*His;C-Myc

Accession

P97429 (E2-D319)

Gene ID
Molecular Construction
N-term
10*His
ANXA4 (E2-D319)
Accession # P97429
Myc
C-term
Synonyms
Anxa4; Anx4Annexin A4; Annexin IV; Annexin-4
AA Sequence

EAKGGTVKAASGFNATEDAQTLRKAMKGLGTDEDAIIGILAYRNTAQRQEIRSAYKSTIGRDLIEDLKSELSSNFEQVILGLMTPTVLYDVQELRRAMKGAGTDEGCLIEILASRTPEEIRRINQTYQQQYGRSLEEDICSDTSFMFQRVLVSLSAAGRDEGNYLDDALMKQDAQELYEAGEKRWGTDEVKFLSILCSRNRNHLLHVFDEYKRISQKDIEQSIKSETSGSFEDALLAIVKCMRSKPSYFAERLYKSMKGLGTDDNTLIRVMVSRAEIDMLDIRASFKRLYGKSLYSFIKGDTSGDYRKVLLVLCGGDD

Molecular Weight

Approximately 40.8 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Annexin A4/ANXA4 Protein, Mouse (His-Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Annexin A4/ANXA4 Protein, Mouse (His-Myc)
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HY-P72079
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