Arginase-1/ARG1 Protein, Human (HEK293, His)

Arginase-1 Protein, Human (HEK293, His) expresses in HEK293 cells with a His tag at the C-terminus. Arginase-1 Protein inhibits the growth of the arginine-depleting enzyme arginine deiminase (ADI)-resistant Hep3B tumor cells in mice^[1].

Recombinant Human Arginase-1 has IC₅₀s of 0.18 U/mL and 0.07 U/mL for HepG2 and Hep3B cells. Recombinant Human Arginase-1 has a K_m value of 1.9 mM for arginine^[1].

Measured by the production of urea during the hydrolysis of arginine. The specific activity is 4.6 ×10⁴-1.5 ×10⁸ pmol/min/µg.

Human

HEK293

C-6*His

P05089 (S2-K322)

383  [NCBI]

SAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPK

Approximately 38.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 20%Glycerol, 1 mM DTT, pH 7.4 or 20 mM Tris-HCl, 150 mM NaCl, 20%Glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Sam-Mui Tsui, et al. Pegylated Derivatives of Recombinant Human Arginase (rhArg1) for Sustained in Vivo Activity in Cancer Therapy: Preparation, Characterization and Analysis of Their Pharmacodynamics in Vivo and in Vitro and Action Upon Hepatocellular Carcinoma Cell (HCC). Cancer Cell Int. 2009 Apr 17;9:9.  [Content Brief]