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  4. Bax Protein, Human (Cell-Free, His)

Bax Protein, Human (Cell-Free, His)

Cat. No.: HY-P702614
COA Handling Instructions

Bax Protein, Human (Cell-Free, His) is the recombinant human-derived Bax, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Bax Protein, Human (Cell-Free, His) is 192 a.a.,

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $550 In-stock
50 μg $1100 In-stock
100 μg   Get quote  
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  • Biological Activity

  • Technical Parameters

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Description

Bax Protein, Human (Cell-Free, His) is the recombinant human-derived Bax, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Bax Protein, Human (Cell-Free, His) is 192 a.a.,
Species

Human
Source

E. coli Cell-free
Tag

N-10*His
Accession

Q07812-1 (M1-G192)
Gene ID

581
Synonyms
rHuBCL2L4, His; Apoptosis Regulator BAX; Bcl-2-Like Protein 4; BCL2L4; BAX
Molecular Weight

23.3 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder
Formulation

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation

COA (206 KB)

Handling Instructions (2659 KB)

Bax Protein, Human (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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