birA Protein, E.coli (His)

The birA protein has the dual role of biotin ligase and biotin operon repressor. In the presence of ATP, it activates biotin to form the BirA-biotinyl-5'-adenylate complex. birA Protein, E.coli (His) is the recombinant E. coli-derived birA protein, expressed by E. coli , with N-6*His labeled tag. The total length of birA Protein, E.coli (His) is 321 a.a., .

The BirA protein serves a dual role as both a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin, leading to the formation of the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can function by transferring the biotinyl group to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, thereby facilitating its activity. Alternatively, holoBirA can bind to the biotin operator site and inhibit the transcription of the operon. Despite its characterization as an orphan receptor, the specific ligand or function of the GPR146 protein remains unidentified.

E.coli

E. coli

N-6*His

Q8X709 (M1-K321)

/

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.