2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. BUB1 Protein, Human (sf9, GST)

BUB1 Protein, Human (sf9, GST)

Cat. No.: HY-P702955
Handling Instructions

BUB1 Protein, Human (sf9, GST) is the recombinant human-derived BUB1, expressed by Sf9 insect cells , with GST labeled tag. The total length of BUB1 Protein, Human (sf9, GST) is 330 a.a.,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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  • Biological Activity

  • Technical Parameters

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Description

BUB1 Protein, Human (sf9, GST) is the recombinant human-derived BUB1, expressed by Sf9 insect cells , with GST labeled tag. The total length of BUB1 Protein, Human (sf9, GST) is 330 a.a.,
Species

Human
Source

Sf9 insect cells
Tag

GST
Accession

O43683-1 (K756-K1085)
Gene ID

699
Synonyms
BUB1L
Molecular Weight

Approximately 63 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

BUB1 Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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