Caspase-14/CASP14 Protein, Human (His)

Caspase-14 Protein, Human (His) is an approximately 31.0 kDa caspase-14 protein with a His-flag. Caspase-14 is a seemingly non-apoptotic caspase involved in keratinocyte differentiation and cornification^[3].

Caspase 14 is a member of the caspase family. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin^[1].
Caspase-14 is a protease that is mainly expressed in suprabasal epidermal layers and activated during keratinocyte cornification^[2].
In contrast to many other caspase family members, murine caspase-14 is not cleaved by granzyme B, caspase-1, caspase-2, caspase-3, caspase-6, caspase-7 or caspase-11, but is weakly processed into p18 and p11 subunits by murine caspase-8^[2].
Caspase-14 is a seemingly non-apoptotic caspase involved in keratinocyte differentiation and cornification. Caspase-14 possesses a conserved role in barrier formation through involvement in terminal differentiation and fusion of the cytotrophoblast^[3].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-6*His

P31944 (S2-Q242)

23581  [NCBI]

SNPRSLEEEKYDMSGARLALILCVTKAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQFQEELEKFQQAIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDPGETVGGDEIVMVIKDSPQTIPTYTDALHVYSTVEGYIAYRHDQKGSCFIQTLVDVFTKRKGHILELLTEVTRRMAEAELVQEGKARKTNPEIQSTLRKRLYLQHHHHHH

Approximately 31.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. S Hu, et al. Caspase-14 is a novel developmentally regulated protease. J Biol Chem  [Content Brief]

  [2]. M Van de Craen, et al. Identification of a new caspase homologue: caspase-14.Cell Death Differ. 1998 Oct;5(10):838-46.  [Content Brief]

  [3]. L White, et al. Caspase-14: a new player in cytotrophoblast differentiation.Reprod Biomed Online. 2007 Mar;14(3):300-7.  [Content Brief]