1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Caspase
  4. Caspase 14
  5. Caspase-14/CASP14 Protein, Human (His)

Caspase-14 Protein, Human (His) is an approximately 31.0 kDa caspase-14 protein with a His-flag. Caspase-14 is a seemingly non-apoptotic caspase involved in keratinocyte differentiation and cornification.

For research use only. We do not sell to patients.

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10 μg USD 165 In-stock
50 μg USD 496 In-stock
100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

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Description

Caspase-14 Protein, Human (His) is an approximately 31.0 kDa caspase-14 protein with a His-flag. Caspase-14 is a seemingly non-apoptotic caspase involved in keratinocyte differentiation and cornification[3].

Background

Caspase 14 is a member of the caspase family. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin[1].
Caspase-14 is a protease that is mainly expressed in suprabasal epidermal layers and activated during keratinocyte cornification[2].
In contrast to many other caspase family members, murine caspase-14 is not cleaved by granzyme B, caspase-1, caspase-2, caspase-3, caspase-6, caspase-7 or caspase-11, but is weakly processed into p18 and p11 subunits by murine caspase-8[2].
Caspase-14 is a seemingly non-apoptotic caspase involved in keratinocyte differentiation and cornification. Caspase-14 possesses a conserved role in barrier formation through involvement in terminal differentiation and fusion of the cytotrophoblast[3].

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

P31944 (S2-Q242)

Gene ID
Molecular Construction
N-term
CASP14 (S2-Q242)
Accession # P31944
6*His
C-term
Synonyms
rHuCaspase-14, His; Caspase-14; CASP-14; CASP14
AA Sequence

SNPRSLEEEKYDMSGARLALILCVTKAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQFQEELEKFQQAIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDPGETVGGDEIVMVIKDSPQTIPTYTDALHVYSTVEGYIAYRHDQKGSCFIQTLVDVFTKRKGHILELLTEVTRRMAEAELVQEGKARKTNPEIQSTLRKRLYLQHHHHHH

Molecular Weight

Approximately 31.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Caspase-14/CASP14 Protein, Human (His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Product Name:
Caspase-14/CASP14 Protein, Human (His)
Cat. No.:
HY-P7743
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