CCN1/Cyr61 Protein, Human (HEK293, Fc)

CCN1/Cyr61 proteins play critical roles in cellular processes, promoting cell proliferation, chemotaxis, angiogenesis, and adhesion. It coordinates wound healing by upregulating key genes related to angiogenesis, inflammation and matrix remodeling in skin fibroblasts and is heparin binding dependent. CCN1/Cyr61 Protein, Human (HEK293, Fc) is the recombinant human-derived CCN1/Cyr61 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CCN1/Cyr61 Protein, Human (HEK293, Fc) is 357 a.a., with molecular weight of 71-80 kDa.

CCN1/Cyr61 protein plays a multifaceted role in cellular processes, actively promoting cell proliferation, chemotaxis, angiogenesis, and adhesion. It demonstrates a crucial involvement in wound healing by orchestrating the up-regulation of key genes related to angiogenesis, inflammation, and matrix remodeling in skin fibroblasts. CCN1's gene-regulatory effects are heparin-binding dependent. Additionally, it exerts regulatory control by down-regulating the expression of collagen type-1 subunits alpha-1 and alpha-2. The protein facilitates cell adhesion and signaling through integrin alpha-6/beta-1, promotes cell migration via integrin alpha-v/beta-5, and stimulates cell proliferation through integrin alpha-v/beta-3. Its interactions with integrins are context-dependent, relying on heparin and the specific cell type. In skin fibroblasts, CCN1 binds ITGA6/ITGB1, in endothelial cells it binds ITGAV/ITGB3, and in platelets, it associates with ITGA2B/ITGB3, thereby influencing diverse cellular functions.

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Recombinant Human CCN1 immobilized at 0.1 μg/mL, 100 μL/well, will mediate 19.19% BALB/3T3 cell adhesion (3 x 10⁴ cell/well added).

Human

HEK293

C-hFc

O00622 (T25-D381)

3491  [NCBI]

TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCDEDSIKDPMEDQDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTTSWSQCSKTCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCLSVKKYRPKYCGSCVDGRCCTPQLTRTVKMRFRCEDGETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFNDIHKFRD

68-80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM Tris-HCl, 6% Trehalose, 4% Mannitol, 0.05% Tween80, pH7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.