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  5. CD28
  6. CD28 Protein, Canine (HEK293, mFc)

CD28 Protein, Canine (HEK293, mFc)

Cat. No.: HY-P77622
SDS COA Handling Instructions

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg $155 In-stock
50 μg $295 In-stock
100 μg   Get quote  
or Bulk Inquiry

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Top Publications Citing Use of Products

  • Technical Parameters

  • Properties

  • Documentation

Species

Canine
Source

HEK293
Tag

C-mFc
Accession

Q9GKP3 (T18-P153)
Gene ID
Molecular Construction
N-term
CD28 (T18-P153)
Accession # Q9GKP3
mFc
C-term
Synonyms
CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
Molecular Weight

50-70 kDa
Purity

Greater than 95% as determined by Tris-Bis PAGE.
Appearance

Lyophilized powder.
Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation
SDS (251 KB)

COA (220 KB)

Handling Instructions (2659 KB)

CD28 Protein, Canine (HEK293, mFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD28 Protein, Canine (HEK293, mFc)
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