2. Recombinant Proteins
  3. CD Antigens Receptor Proteins
  4. Macrophage CD Proteins Monocyte CD Proteins Stem Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  5. CD31/PECAM-1 Immunoglobulin-like Cell Adhesion Molecules
  6. CD31/PECAM-1 Protein, Cynomolgus (HEK293, His)

CD31/PECAM-1 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P700675
Handling Instructions

The CD31/PECAM-1 protein is a cell adhesion molecule critical for leukocyte transendothelial migration (TEM) under inflammatory conditions. CD31/PECAM-1 also regulates bradykinin receptor BDKRB2 activation and modulates ERK1/2 activation in endothelial cells. CD31/PECAM-1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD31/PECAM-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD31/PECAM-1 Protein, Cynomolgus (HEK293, His) is 575 a.a., with molecular weight of 95-115 kDa.

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Description

The CD31/PECAM-1 protein is a cell adhesion molecule critical for leukocyte transendothelial migration (TEM) under inflammatory conditions. CD31/PECAM-1 also regulates bradykinin receptor BDKRB2 activation and modulates ERK1/2 activation in endothelial cells. CD31/PECAM-1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD31/PECAM-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD31/PECAM-1 Protein, Cynomolgus (HEK293, His) is 575 a.a., with molecular weight of 95-115 kDa.
Background

CD31/PECAM-1 protein, a pivotal cell adhesion molecule, is essential for leukocyte transendothelial migration (TEM) in most inflammatory conditions. The critical role of Tyr-679 in TEM is underscored by its requirement for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC), crucial for targeting the LBRC membrane around migrating leukocytes. The trans-homophilic interaction potentially contributes to endothelial cell-cell adhesion via cell junctions, while heterophilic interaction with CD177 plays a role in the transendothelial migration of neutrophils. Homophilic ligation of PECAM1 serves a dual purpose, preventing macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal, while promoting the macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to phagocytic cells. Notably, PECAM1 modulates bradykinin receptor BDKRB2 activation and regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Its interaction with various partners, including BDKRB2, GNAQ, PTPN11, FER, and CD177, further elucidates the intricate molecular mechanisms governing its diverse cellular functions, while its trans-homodimerization is crucial for effective cell-cell interaction. The interaction nuances, such as Ca(2+)-dependency and directness, add layers of complexity to the regulatory roles of CD31/PECAM-1.
Species

Cynomolgus
Source

HEK293
Tag

C-His
Accession

A0A7N9CHZ3 (H28-G602)
Gene ID

/
Molecular Construction
N-term
CD31 (H28-G602)
Accession # A0A7N9CHZ3
His
C-term
Synonyms
PECAM-1; EndoCAM; PECA1; CD31; FLJ34100; FLJ58394; GPIIA'
Molecular Weight

95-115 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CD31/PECAM-1 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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