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  2. CD Antigens Complement System
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  4. Membrane Cofactor Protein/CD46
  5. CD46 Protein, Human (HEK293, hFc)

CD46 Protein, Human (HEK293, hFc)

Cat. No.: HY-P72020
COA Handling Instructions

CD46 Protein serves as a cofactor for complement factor I, safeguarding cells against complement-induced harm. It aids in sperm-oocyte fusion and acts as a T-cell costimulatory factor, facilitating the formation of T-regulatory 1 cells. These cells secrete interleukin-10, suppressing immune responses and preventing autoimmunity. Pathogens can exploit CD46's immune regulation abilities to induce an immunosuppressive state in T-cells. CD46 Protein, Human (HEK293, hFc) is the recombinant human-derived CD46 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.

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10 μg $90 In-stock
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Description

CD46 Protein serves as a cofactor for complement factor I, safeguarding cells against complement-induced harm. It aids in sperm-oocyte fusion and acts as a T-cell costimulatory factor, facilitating the formation of T-regulatory 1 cells. These cells secrete interleukin-10, suppressing immune responses and preventing autoimmunity. Pathogens can exploit CD46's immune regulation abilities to induce an immunosuppressive state in T-cells. CD46 Protein, Human (HEK293, hFc) is the recombinant human-derived CD46 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

CD46 Protein acts as a cofactor for complement factor I, protecting cells from complement-mediated injury by cleaving C3b and C4b on host tissue. It also plays a role in sperm-oocyte fusion during fertilization and acts as a costimulatory factor for T-cells, promoting the differentiation of CD4+ cells into T-regulatory 1 cells. These T-regulatory 1 cells suppress immune responses by secreting interleukin-10, thus preventing autoimmunity. In the context of microbial infection, certain viral and bacterial pathogens exploit CD46's immune regulation properties by binding to it and inducing an immunosuppressive phenotype in T-cells.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/mL can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8-1.38 ng/mL.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

P15529-1 (C35-Y328)

Gene ID
Molecular Construction
N-term
CD46 (C35-Y328)
Accession # P15529-1
hFc
C-term
Synonyms
AHUS2; Antigen defined by monoclonal TRA 2 10; Antigen identified by monoclonal TRA 2 10; CD46; TRA2.10
AA Sequence

CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY

Molecular Weight

Approximately 70.0 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD46 Protein, Human (HEK293, hFc)
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HY-P72020
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