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  4. Ig-alpha/CD79a Ig-β/CD79b
  5. CD79 Protein, Human (HEK293, His)

The CD79A protein is critical for initiating the B cell antigen receptor complex (BCR) signaling cascade upon antigen binding. It promotes internalization, transport to late endosomes, and antigen presentation of BCR complexes. CD79 Protein, Human (HEK293, His) is the recombinant human-derived CD79, expressed by HEK293 , with C-6*His labeled tag. ,

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Description

The CD79A protein is critical for initiating the B cell antigen receptor complex (BCR) signaling cascade upon antigen binding. It promotes internalization, transport to late endosomes, and antigen presentation of BCR complexes. CD79 Protein, Human (HEK293, His) is the recombinant human-derived CD79, expressed by HEK293 , with C-6*His labeled tag. ,

Background

The CD79A protein is essential for the initiation of the signal transduction cascade in response to antigen binding to the B-cell antigen receptor complex (BCR). It plays a crucial role in internalizing the BCR complex, trafficking it to late endosomes, and facilitating antigen presentation. CD79A is also necessary for the surface expression of the BCR and efficient differentiation of pro- and pre-B-cells. It promotes the autophosphorylation and activation of SYK, a key signaling molecule, by binding to BLNK and bringing it into proximity with SYK, allowing for the phosphorylation of BLNK. CD79A also interacts with certain Src-family tyrosine kinases, such as FYN and LYN, increasing their activity. Notably, it represses BCR signaling during the development of immature B-cells. CD79A forms a disulfide-linked heterodimer with CD79B, and together they constitute the B-cell antigen receptor complex, where the alpha/beta chain heterodimer is non-covalently associated with an antigen-specific membrane-bound surface immunoglobulin composed of two heavy chains and two light chains. CD79A interacts with SYK through its phosphorylated ITAM domain, facilitating SYK autophosphorylation and activation. Additionally, when phosphorylated on Tyr-210, CD79A interacts with the SH2 domain of BLNK/SLP65, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK, which is necessary for the trafficking of the BCR to late endosomes.

Biological Activity

Immobilized Human CD79 at 1 μg/mL (100 μL/well) can bind Anti-CD79B antibody. The ED50 for this effect is 21.28 ng/mL.

  • Immobilized Human CD79 at 1 μg/mL (100 μL/well) can bind Anti- CD79B antibody. The ED50 for this effect is 21.28 ng/mL.
Species

Human

Source

HEK293

Tag

C-6*His

Accession

P11912-1 (L33-R143) & P40259-1 (A29-D159)

Gene ID

973 & 974

Synonyms
B-cell antigen receptor complex-associated protein; CD79a; IGA; MB1; CD79b; IGB
Molecular Weight

Approximately 25-55 kDa due to the glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD79 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD79 Protein, Human (HEK293, His)
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HY-P75386A
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