CD96 Protein, Human (HEK293, mFc)

CD96 promotes adhesive interactions between activated T cells and NK cells in the late stages of the immune response. It promotes the adhesion between NK cells and target cells by interacting with PVR on target cells. CD96 Protein, Human (HEK293, mFc) is the recombinant human-derived CD96 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of CD96 Protein, Human (HEK293, mFc) is 482 a.a., with molecular weight of 120-160 kDa.

CD96 protein appears to play a role in adhesive interactions during the late phase of the immune response, potentially involving activated T and NK cells. It facilitates NK cell-target adhesion by interacting with PVR present on target cells. CD96 may function in a phase after T and NK cells have penetrated the endothelium using integrins and selectins, actively engaging with diseased cells and navigating within areas of inflammation. Structurally, CD96 forms homodimers through disulfide linkages and interacts specifically with PVR, suggesting its involvement in cell-cell interactions crucial for immune responses during inflammation and infection.

1. Immobilized Human CD96, mFc Tag at 5 μg/mL (100 μl/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC₅₀ of 0.21 μg/mL determined by ELISA.
2. Immobilized Human CD96, mFc Tag at 2 μg/mL (100 μl/Well) on the plate. Dose response curve for Anti-CD96 Antibody, hFc Tag with the EC₅₀ of ≤25.9 ng/mL determined by ELISA.

Human

HEK293

C-mFc

P40200-2 (V22-M503)

10225  [NCBI]

120-160 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.