CHEK2 Protein, Human (sf9, GST)

The CHEK2 protein is a serine/threonine protein kinase that critically coordinates checkpoint-mediated cell cycle arrest, DNA repair activation, and apoptosis in response to DNA double-strand breaks. Activated CHEK2 phosphorylates CDC25A, CDC25B, and CDC25C, inhibiting their phosphatase activities and preventing the CDK-cyclin complex from activating cell cycle progression. CHEK2 Protein, Human (sf9, GST) is the recombinant human-derived CHEK2 protein, expressed by Sf9 insect cells , with GST labeled tag.

The CHEK2 protein is a serine/threonine-protein kinase that plays a crucial role in checkpoint-mediated cell cycle arrest, DNA repair activation, and apoptosis in response to DNA double-strand breaks. It also plays a role in negatively regulating cell cycle progression during normal cell cycles. Upon activation, CHEK2 phosphorylates various effectors at a specific consensus sequence, inhibiting the activity of CDC25A, CDC25B, and CDC25C. This inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, preventing cell cycle progression. CHEK2 also phosphorylates NEK6, involved in G2/M cell cycle arrest, and BRCA2, enhancing the association of RAD51 with chromatin for DNA repair through homologous recombination. Furthermore, CHEK2 stimulates the transcription of DNA repair genes, including BRCA2, by phosphorylating and activating the transcription factor FOXM1. In terms of apoptosis, CHEK2 phosphorylates p53/TP53, MDM4, and PML, leading to the accumulation of active p53/TP53 and reduced degradation of p53/TP53. It also phosphorylates the transcription factor E2F1 to control the transcription of pro-apoptotic genes. Additionally, CHEK2 has a role in mitotic spindle assembly by phosphorylating BRCA1, independent of DNA damage. Its absence may contribute to the chromosomal instability observed in certain cancer cells. CHEK2 also plays a role in microbial infection by phosphorylating the herpes simplex virus 1/HHV-1 protein ICP0 to activate its SUMO-targeted ubiquitin ligase activity.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.The Km of CHEK2 is 201.7 μM.

Human

Sf9 insect cells

N-GST

O96017-1 (S2-L543)

11200  [NCBI]

87.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M Trehalose, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.