CLEC9A Protein, Human (HEK293, Fc)

The CLEC9A Protein, expressed on a specific subset of myeloid cells, efficiently uptakes and processes material from deceased cells. It recognizes filamentous actin, especially with actin-binding domains like spectrin, when cell membranes are compromised. This facilitates Syk-dependent cross-presentation of antigens from dead cells. CLEC9A functions as a homodimer, highlighting its role in specialized endocytic processes of myeloid cells. CLEC9A Protein, Human (HEK293, Fc) is the recombinant human-derived CLEC9A protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CLEC9A Protein, Human (HEK293, Fc) is 185 a.a., with molecular weight of 55-65 kDa.

CLEC9A Protein operates as an endocytic receptor uniquely expressed on a specific subset of myeloid cells, designed for the efficient uptake and processing of material derived from deceased cells. This receptor exhibits a distinct recognition capability for the filamentous form of actin, particularly in conjunction with specific actin-binding domains found in cytoskeletal proteins like spectrin. This recognition occurs when cell membranes are compromised, facilitating the cross-presentation of antigens associated with dead cells in a Syk-dependent manner. Notably, CLEC9A functions as a homodimer, emphasizing its role in the specialized endocytic processes performed by this subset of myeloid cells.

Immobilized Human CLEC9A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC₅₀ of 4.4ng/ml determined by ELISA.

Human

HEK293

N-hFc

Q6UXN8 (K57-V241)

283420  [NCBI]

55-65 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.