Coagulation Factor II/F2 Protein, Rat (HEK293, His)

The factor II/F2 protein (also known as thrombin) plays a key role in blood homeostasis, inflammation, and wound healing. As a multifunctional enzyme, it catalyzes the conversion of fibrinogen into fibrin to form the coagulation network. Coagulation Factor II/F2 Protein, Rat (HEK293, His) is the recombinant rat-derived Coagulation Factor II/F2 protein, expressed by HEK293 , with C-His labeled tag.

Coagulation Factor II/F2 Protein, commonly known as thrombin, plays a pivotal role in blood homeostasis, inflammation, and wound healing. This multifunctional enzyme, with its ability to cleave bonds after Arg and Lys, serves as a key player in the coagulation cascade. Thrombin catalyzes the conversion of fibrinogen to fibrin, forming the essential meshwork for blood clotting. Additionally, it activates several factors including V, VII, VIII, XIII, and, when in complex with thrombomodulin, protein C, contributing to the intricate regulation of coagulation and anticoagulation pathways. The diverse functions of Coagulation Factor II/F2 underscore its indispensable role in maintaining the delicate balance of blood clotting, immune responses, and the intricate processes involved in tissue repair and healing.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Rat

HEK293

C-His

P18292/NP_075213.2 (Q25-R617)

29251  [NCBI]

QHVFLAPQQALSLLQRVRRANSGFLEELRKGNLERECVEEQCSYEEAFEALESPQDTDVFWAKYTVCDSVRKPRETFMDCLEGRCAMDLGLNYHGNVSVTHTGIECQLWRSRYPHRPDINSTTHPGADLKENFCRNPDSSTSGPWCYTTDPTVRREECSIPVCGQEGRTTVKMTPRSRGSKENLSPPLGECLLERGRLYQGNLAVTTLGSPCLAWDSLPTKTLSKYQNFDPEVKLVQNFCRNPDRDEEGAWCFVAQQPGFEYCSLNYCDEAVGEENHDGDESIAGRTTDAEFHTFFDERTFGLGEADCGLRPLFEKKSLTDKTEKELLDSYIDGRIVEGWDAEKGIAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLLVRIGKHSRTRYERNVEKISMLEKIYIHPRYNWRENLDRDIALLKLKKPVPFSDYIHPVCLPDKQTVTSLLQAGYKGRVTGWGNLRETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMFCAGFKVNDTKRGDACEGDSGGPFVMKSPYNHRWYQMGIVSWGEGCDRNGKYGFYTHVFRLKRWMQKVIDQHR

Approximately 69.5 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.