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Collagen alpha-1(IX) chain/COL9A1 Protein, Human (HEK293, His)

Cat. No.: HY-P70009
Handling Instructions

Collagen alpha-1(IX) chain (COL9A1) is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage and a structural component of vitreous in the eye. Synthesis of COL9A1 chain is essential for assembly of type IX collagen molecules, and mutations of COL9A1 are associated with osteoarthritis in humans, multiple epiphyseal dysplasia, and Stickler syndrome. Collagen alpha-1 (IX) chain/COL9A1 Protein, Human (HEK293, His) is the recombinant human-derived Collagen alpha-1(IX) chain/COL9A1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Collagen alpha-1 (IX) chain/COL9A1 Protein, Human (HEK293, His) is 575 a.a., with molecular weight of 38-50 kDa.

For research use only. We do not sell to patients.

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Description

Collagen alpha-1(IX) chain (COL9A1) is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage and a structural component of vitreous in the eye. Synthesis of COL9A1 chain is essential for assembly of type IX collagen molecules, and mutations of COL9A1 are associated with osteoarthritis in humans, multiple epiphyseal dysplasia, and Stickler syndrome. Collagen alpha-1 (IX) chain/COL9A1 Protein, Human (HEK293, His) is the recombinant human-derived Collagen alpha-1(IX) chain/COL9A1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Collagen alpha-1 (IX) chain/COL9A1 Protein, Human (HEK293, His) is 575 a.a., with molecular weight of 38-50 kDa.

Background

Collagen alpha-1(IX) chain (COL9A1) is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage and a structural component of vitreous in the eye. COL9A1 has two isoforms through alternative slice. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Synthesis of COL9A1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in COL9A1 are associated with osteoarthritis in humans, multiple epiphyseal dysplasia, and Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects[1][2][3][4].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P20849-3 (A24-G598)

Gene ID
Molecular Construction
N-term
COL9A1 (A24-G598)
Accession # P20849-3
6*His
C-term
Synonyms
rHuCollagen alpha-1(IX) chain/COL9A1, His; Collagen alpha-1(IX) chain; DJ149L1.1.2; EDM6; MED; STL4
AA Sequence

AVKRRPRFPVNSNSNGGNELCPKIRIGQDDLPGFDLISQFQVDKAASRRAIQRVVGSATLQVAYKLGNNVDFRIPTRNLYPSGLPEEYSFLTTFRMTGSTLKKNWNIWQIQDSSGKEQVGIKINGQTQSVVFSYKGLDGSLQTAAFSNLSSLFDSQWHKIMIGVERSSATLFVDCNRIESLPIKPRGPIDIDGFAVLGKLADNPQVSVPFELQWMLIHCDPLRPRRETCHELPARITPSQTTDERGPPGEQGPPGPPGPPGVPGIDGIDGDRGPKGPPGPPGPAGEPGKPGAPGKPGTPGADTSP

Molecular Weight

38-50 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Collagen alpha-1(IX) chain/COL9A1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Collagen alpha-1(IX) chain/COL9A1 Protein, Human (HEK293, His)
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HY-P70009
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